Thirty-five Staphylococcus aureus strains from auricular infections were isolated. The identification of strains was confirmed by Api ID 32 Staph strips, the antibiotic susceptibility test was performed using ATB Staph kit. PCR assay was used to detect the oxacillin resistance gene (mecA) and the erythromycin genes (ermA, ermB, ermC, msrA and mef). The susceptibility profile of all strains revealed a low resistance level to oxacillin and erythromycin. The PCR results show that 60 % of the strains are mecA positive. The frequency of erythromycin genes was: ermA (+) 22.8 %, ermB (+) 45.7, ermC (+) 17.1, msrA (+) 28.6. The mef gene was not detected in any strain. No correlations between genotypic and phenotypic methods for the determination of oxacillin and erythromycin resistance was found. However, multiplex PCR technique was shown to be a fast, practical and economic technique for the detection of methicillin-and erythromycin-resistant staphylococci.

Laboratoire d'Analyse Traitement et Valorisation des Polluants de l'Environnement et des Produits Faculté de Pharmacie Monastir Tunisia

Zobrazit více v PubMed

Diagn Microbiol Infect Dis. 1998 Mar;30(3):205-14 PubMed

Jpn J Infect Dis. 2004 Apr;57(2):74-7 PubMed

J Hosp Infect. 2002 May;51(1):33-42 PubMed

Gene. 1995 Feb 3;153(1):93-8 PubMed

Folia Microbiol (Praha). 2007;52(3):281-5 PubMed

Diagn Microbiol Infect Dis. 2002 Jul;43(3):225-32 PubMed

Infect Control Hosp Epidemiol. 1995 Jan;16(1):12-7 PubMed

Antimicrob Agents Chemother. 1999 May;43(5):1062-6 PubMed

Antimicrob Agents Chemother. 1995 Mar;39(3):577-85 PubMed

Mol Microbiol. 1990 Jul;4(7):1207-14 PubMed

Diagn Microbiol Infect Dis. 1999 Apr;33(4):283-97 PubMed

J Antimicrob Chemother. 1993 Feb;31(2):211-7 PubMed

J Clin Microbiol. 2003 Sep;41(9):4089-94 PubMed

Pathol Biol (Paris). 1998 Apr;46(4):227-34 PubMed

Clin Microbiol Rev. 2001 Oct;14(4):836-71, table of contents PubMed

Folia Microbiol (Praha). 2006;51(3):236-8 PubMed

Antimicrob Agents Chemother. 1996 Nov;40(11):2562-6 PubMed

Arch Med Res. 2006 Nov;37(8):953-60 PubMed

J Clin Microbiol. 2002 Mar;40(3):774-8 PubMed

Clin Microbiol Rev. 1997 Oct;10(4):781-91 PubMed

J Clin Microbiol. 1994 Jul;32(7):1768-72 PubMed

Folia Microbiol (Praha). 2007;52(6):593-9 PubMed

Clin Infect Dis. 2002 Feb 15;34(4):482-92 PubMed

Antimicrob Agents Chemother. 2000 Feb;44(2):231-8 PubMed

J Antimicrob Chemother. 2002 Mar;49(3):489-95 PubMed

J Antimicrob Chemother. 1999 Sep;44(3):351-8 PubMed

Int J Antimicrob Agents. 2005 Sep;26(3):213-8 PubMed

Antimicrobial resistance and presence of ileS-2 gene encoding mupirocin resistance in clinical isolates of methicillin-resistant Staphylococcus sp