Age-related changes in GAD levels in the central auditory system of the rat
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18930128
DOI
10.1016/j.exger.2008.09.012
PII: S0531-5565(08)00320-3
Knihovny.cz E-resources
- MeSH
- Analysis of Variance MeSH
- Inferior Colliculi metabolism MeSH
- Species Specificity MeSH
- gamma-Aminobutyric Acid metabolism physiology MeSH
- Glutamate Decarboxylase metabolism MeSH
- Rats MeSH
- Humans MeSH
- Rats, Inbred F344 MeSH
- Rats, Long-Evans MeSH
- Presbycusis metabolism MeSH
- Protein Isoforms analysis metabolism MeSH
- Auditory Cortex metabolism MeSH
- Aging metabolism MeSH
- Blotting, Western methods MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- gamma-Aminobutyric Acid MeSH
- Glutamate Decarboxylase MeSH
- Protein Isoforms MeSH
Changes in the levels of gamma-aminobutyric acid (GABA) are known to occur in different parts of the brain during aging. In our study we attempted to define the effect that aging has on glutamate decarboxylase (GAD), the key enzyme in the synthesis of GABA, in the central parts of the auditory system. Age-related changes in GAD65 and GAD67 levels were investigated using immunohistochemistry and Western blotting in the inferior colliculus (IC), the auditory cortex (AC) and the visual cortex in Long-Evans rats. The results show that aging is associated with a decrease in the numbers of GAD65- and 67-immunoreactive neurons and the optical density of their somas in both the IC and AC. Western blot analysis revealed a pronounced age-related decline in the levels of GAD65 and 67 proteins in both the IC and AC. For comparison, in the visual cortex the decrease in both proteins was less pronounced than in the IC and AC. A similar pattern of age-related changes was found in Fischer 344 rats, a strain that manifests a rapid loss of hearing function with aging. The observed age-related decline in the levels of GAD65 and 67 may contribute significantly to the deterioration of hearing function that accompanies aging in mammals, including man.
References provided by Crossref.org
Functional changes in the human auditory cortex in ageing
Immunocytochemical profiles of inferior colliculus neurons in the rat and their changes with aging
