Molecular detection of Borrelia bissettii DNA in serum samples from patients in the Czech Republic with suspected borreliosis
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19187198
DOI
10.1111/j.1574-6968.2009.01498.x
PII: FML1498
Knihovny.cz E-resources
- MeSH
- RNA, Bacterial genetics MeSH
- Borrelia genetics isolation & purification MeSH
- DNA, Bacterial blood chemistry genetics MeSH
- Phylogeny MeSH
- Genes, rRNA MeSH
- Borrelia Infections diagnosis MeSH
- Humans MeSH
- Molecular Sequence Data MeSH
- Polymerase Chain Reaction methods MeSH
- DNA, Ribosomal chemistry genetics MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sequence Analysis, DNA MeSH
- Sequence Homology, Nucleic Acid MeSH
- Serum microbiology MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- RNA, Bacterial MeSH
- DNA, Bacterial MeSH
- DNA, Ribosomal MeSH
- RNA, Ribosomal, 16S MeSH
Until recently, three spirochete genospecies were considered to be the causative agents of Lyme borreliosis (LB) in Europe: Borrelia burgdorferi sensu stricto, Borrelia afzelii and Borrelia garinii. However, the DNA of Borrelia valaisiana, Borrelia lusitaniae, Borrelia spielmanii and Borrelia bissettii has already been detected in samples of human origin, or the spirochetes were isolated from the patients with symptoms of LB. Molecular analysis of 12 selected serum samples collected in the regional hospital confirmed the presence of B. bissettii DNA in cases of single and multiple infection in patients with symptomatic borreliosis or chronic borrelial infection. The presence of B. bissettii as a single strain in patients provides strong support of the fact that B. bissettii might be a causative agent of the disease. After the first isolation of B. bissettii from the samples of human origin in Slovenia, following the detection of this species in cardiac valve tissue of the patient with endocarditis and aortic valve stenosis in the Czech Republic, here we present additional molecular data supporting the involvement of B. bissettii in LB in Europe.
References provided by Crossref.org
Pathogenicity and virulence of Borrelia burgdorferi
Sexual Transmission of Lyme Borreliosis? The Question That Calls for an Answer
Comparative study of binding of ovine complement factor H with different Borrelia genospecies
Updates on Borrelia burgdorferi sensu lato complex with respect to public health
GENBANK
FJ231330, FJ231331, FJ231332, FJ231333, FJ231334, FJ231335, FJ231336, FJ231337, FJ231338, FJ231339, FJ231340, FJ231341, FJ231342, FJ231343, FJ231344, FJ231345, FJ231346, FJ431126, FJ431127, FJ431128, FJ431129, FJ431130, FJ431131, FJ431132, FJ431133, FJ431134, FJ431135, FJ431136, FJ431137, FJ431138, FJ431139, FJ431140, FJ431141, FJ431142
