Development and activity of Glomus intraradices as affected by co-existence with Glomus claroideum in one root system
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19377892
DOI
10.1007/s00572-009-0243-4
PII: 10.1007/s00572-009-0243-4
Knihovny.cz E-resources
- MeSH
- Alkaline Phosphatase metabolism MeSH
- DNA Primers genetics MeSH
- Genes, Fungal MeSH
- Genes, rRNA MeSH
- Glomeromycota growth & development MeSH
- Plant Roots microbiology MeSH
- Poaceae growth & development microbiology MeSH
- Polymerase Chain Reaction methods MeSH
- Gene Expression Profiling MeSH
- Tripleurospermum growth & development microbiology MeSH
- Trypan Blue metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Europe MeSH
- Names of Substances
- Alkaline Phosphatase MeSH
- DNA Primers MeSH
- Trypan Blue MeSH
The co-existence of two arbuscular mycorrhizal fungal (AMF) species, Glomus intraradices and Glomus claroideum, in the root systems of plants was investigated in a greenhouse experiment aimed at reconstructing interactions during an early stage of primary succession on a coal-mine spoil bank in Central Europe. Two plant species, Tripleurospermum inodorum and Calamagrostis epigejos, were inoculated either with one or both AMF species. Fungal development, determined by trypan blue and alkaline phosphatase staining as well as by PCR amplification of rRNA genes with species-specific primers, and the expression of five genes with different metabolic functions in the intraradical structures of G. intraradices were followed after 6 and 9 weeks of cultivation. The two AMF closely co-existed in the root systems of both plants possibly through similar colonisation rates and competitivity. Inoculation with the two fungi, however, did not bring any additional benefit to the host plants in comparison with single inoculation; moreover, plant growth depression observed after inoculation with G. claroideum persisted also in mixed inoculation. The expression of all the assayed G. intraradices genes was affected either by host plant or by co-inoculation with G. claroideum. The effects of both factors depended on the time of sampling, which underlines the importance of addressing this topic in time-course studies.
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