Study of the photodynamic effect on the A549 cell line by atomic force microscopy and the influence of green tea extract on the production of reactive oxygen species
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19723103
DOI
10.1111/j.1749-6632.2009.04730.x
PII: NYAS4730
Knihovny.cz E-resources
- MeSH
- Apoptosis drug effects MeSH
- Tea chemistry MeSH
- Photochemotherapy methods MeSH
- Photosensitizing Agents pharmacology MeSH
- Oxygen metabolism pharmacology MeSH
- Humans MeSH
- Metalloporphyrins pharmacology MeSH
- Microscopy, Atomic Force methods MeSH
- Cell Line, Tumor MeSH
- Lung Neoplasms metabolism pathology MeSH
- Reactive Oxygen Species metabolism MeSH
- Plant Extracts pharmacology MeSH
- Light MeSH
- Cell Survival drug effects MeSH
- Dose-Response Relationship, Drug MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Tea MeSH
- Photosensitizing Agents MeSH
- Oxygen MeSH
- Metalloporphyrins MeSH
- Reactive Oxygen Species MeSH
- Plant Extracts MeSH
- zinc-tetrakis(p-sulfonatophenyl)porphyrin MeSH Browser
We studied the morphology of the A549 cell line (human lung carcinoma cells) before and after photodynamic therapy (PDT) by atomic force microscopy. PDT was induced by an efficient light-emitting diode source with total light dose of 15 J cm(-2) in the presence of the sensitizer zinc-5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrine. In the presence of molecular oxygen, light activation of the photosensitizer, which accumulates in cancer cells, leads to the local production of reactive oxygen species (ROS). This is one of several reasons leading to cell death, and in some cases we could observe signs of apoptosis. We detected the kinetics of ROS production to be dependent on the presence of green tea extract.
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