Selection of reference genes for real-time polymerase chain reaction analysis in tissues from Bombus terrestris and Bombus lucorum of different ages
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Technical Report
PubMed
19751695
DOI
10.1016/j.ab.2009.09.019
PII: S0003-2697(09)00642-3
Knihovny.cz E-resources
- MeSH
- Actins genetics MeSH
- Arginine Kinase genetics MeSH
- Peptide Elongation Factor 1 genetics MeSH
- Exocrine Glands metabolism MeSH
- Phospholipases A2 genetics MeSH
- Glyceraldehyde-3-Phosphate Dehydrogenases genetics MeSH
- Polymerase Chain Reaction methods MeSH
- Ribosomal Proteins genetics MeSH
- Tubulin genetics MeSH
- Fat Body metabolism MeSH
- Bees genetics MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Technical Report MeSH
- Names of Substances
- Actins MeSH
- Arginine Kinase MeSH
- Peptide Elongation Factor 1 MeSH
- Phospholipases A2 MeSH
- Glyceraldehyde-3-Phosphate Dehydrogenases MeSH
- Ribosomal Proteins MeSH
- Tubulin MeSH
Quantitative real-time polymerase chain reaction (PCR) is an accurate and sensitive technique for gene expression analysis. However, it requires data normalization using reference genes. Here we assessed the stability of eight reference genes in the labial gland and fat body of the bumblebees Bombus terrestris and Bombus lucorum of different ages. To date, no reference genes have been identified for these species. Our data show that arginine kinase (AK) and phospholipase A2 (PLA2) are the most stable genes in both tissues of B. terrestris. The most stable genes for the labial gland and fat body of B. lucorum were found to be elongation factor 1alpha (EEF1A) and PLA2.
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