The positive effect of immunosuppression on adaptation of venous allografts to arterialisation in rats
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20015668
DOI
10.1016/j.ejvs.2009.11.016
PII: S1078-5884(09)00585-1
Knihovny.cz E-resources
- MeSH
- Aorta, Abdominal surgery MeSH
- Time Factors MeSH
- CD4-Positive T-Lymphocytes drug effects immunology MeSH
- CD8-Positive T-Lymphocytes drug effects immunology MeSH
- Endothelial Cells drug effects pathology MeSH
- Neovascularization, Physiologic drug effects MeSH
- Transplantation, Homologous MeSH
- Immunosuppressive Agents administration & dosage pharmacology MeSH
- Injections, Intramuscular MeSH
- Rats MeSH
- Models, Animal MeSH
- Cell Movement drug effects MeSH
- Rats, Inbred BN MeSH
- Rats, Inbred Lew MeSH
- Graft Survival drug effects MeSH
- Cell Proliferation drug effects MeSH
- Graft Rejection immunology pathology prevention & control MeSH
- Muscle, Smooth, Vascular drug effects pathology MeSH
- Tacrolimus administration & dosage pharmacology MeSH
- Veins drug effects immunology pathology transplantation MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Immunosuppressive Agents MeSH
- Tacrolimus MeSH
OBJECTIVES AND DESIGN: We investigated whether immunosuppression was necessary for transplanted allogeneic veins to adapt to arterialisation. We used a transplant rat model with or without immunosuppression. MATERIAL AND METHODS: Iliolumbar veins from Lewis (LEW) or Brown-Norway (BN) rats were transplanted into the abdominal aorta of isogeneic (LEW to LEW; group A) or allogeneic (BN to LEW; groups B and C) rats. Group C had daily intramuscular injections of 0.2mgkg(-1) FK506. Light microscope evaluations of grafts were performed at 30 days following transplantation. We determined the presence of endothelial cells, the intensity of intimal proliferation and the degree of infiltration by Lewis major histocompatibility complex (MHC) class II positive, CD4-positive and CD8-positive cells into the adventitia. RESULTS: Groups A and C displayed similar results in intimal thickness (12.7+/-7.0microm vs. 15.0+/-8.4 mum, respectively) and degree of adventitial infiltration by MHC class II positive (16.6+/-7.5 vs. 14.6+/-6.2, respectively), CD8-positive (0.8+/-1.7 vs. 1.8+/-2.6, respectively) and CD4-positive (12.5+/-7.7 vs. 5.8+/-4.6, respectively) cells. In contrast, allogeneic rats without immunosuppression (group B) showed infiltration of host immunocompetent cells and destruction of the venous wall with no histological signs of arterialisation. CONCLUSION: Immunosuppressive therapy is necessary for venous allograft adaptation to arterialisation in rats.
References provided by Crossref.org
