HPMA-RGD hydrogels seeded with mesenchymal stem cells improve functional outcome in chronic spinal cord injury
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20053128
DOI
10.1089/scd.2009.0378
Knihovny.cz E-resources
- MeSH
- Behavior, Animal physiology MeSH
- Chronic Disease MeSH
- Implants, Experimental MeSH
- Hydrogels chemistry MeSH
- Rats MeSH
- Humans MeSH
- Methacrylates chemistry MeSH
- Mesenchymal Stem Cells * cytology physiology MeSH
- Spinal Cord pathology MeSH
- Random Allocation MeSH
- Oligopeptides chemistry MeSH
- Spinal Cord Injuries pathology therapy MeSH
- Rats, Wistar MeSH
- Nerve Regeneration physiology MeSH
- Mesenchymal Stem Cell Transplantation * MeSH
- Treatment Outcome MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- arginyl-glycyl-aspartic acid MeSH Browser
- Hydrogels MeSH
- hydroxypropyl methacrylate MeSH Browser
- Methacrylates MeSH
- Oligopeptides MeSH
Chronic spinal cord injury (SCI) is characterized by tissue loss and a stable functional deficit. While several experimental therapies have proven to be partly successful for the treatment of acute SCI, treatment of chronic SCI is still challenging. We studied whether we can bridge a chronic spinal cord lesion by implantation of our newly developed hydrogel based on 2-hydroxypropyl methacrylamide, either alone or seeded with mesenchymal stem cells (MSCs), and whether this treatment leads to functional improvement. A balloon-induced compression lesion was performed in adult 2-month-old male Wistar rats. Five weeks after injury, HPMA-RGD hydrogels [N-(2-hydroxypropyl)-methacrylamide with attached amino acid sequences--Arg-Gly-Asp] were implanted into the lesion, either with or without seeded MSCs. Animals with chronic SCI served as controls. The animals were behaviorally tested using the Basso–Beattie-Breshnahan (BBB) (motor) and plantar (sensory) tests once a week for 6 months. Behavioral analysis showed a statistically significant improvement in rats with combined treatment, hydrogel and MSCs, compared with the control group (P < 0.05). Although a tendency toward improvement was found in rats treated with hydrogel only, this was not significant. Subsequently, the animals were sacrificed 6 months after SCI, and the spinal cord lesions evaluated histologically. The combined therapy (hydrogel with MSCs) prevented tissue atrophy (P < 0.05), and the hydrogels were infiltrated with axons myelinated with Schwann cells. Blood vessels and astrocytes also grew inside the implant. MSCs were present in the hydrogels even 5 months after implantation. We conclude that 5 weeks after injury, HPMA-RGD hydrogels seeded with MSCs can successfully bridge a spinal cord cavity and provide a scaffold for tissue regeneration. This treatment leads to functional improvement even in chronic SCI.
References provided by Crossref.org
Mesenchymal Stem Cells in Treatment of Spinal Cord Injury and Amyotrophic Lateral Sclerosis
Biomaterials and Magnetic Stem Cell Delivery in the Treatment of Spinal Cord Injury
Modified Methacrylate Hydrogels Improve Tissue Repair after Spinal Cord Injury
Human mesenchymal stem cells modulate inflammatory cytokines after spinal cord injury in rat
Stem cells in regenerative medicine
