Controlled dehydration of a biological sample using an alternative form of environmental SEM
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20055914
DOI
10.1111/j.1365-2818.2009.03216.x
PII: JMI3216
Knihovny.cz E-resources
- MeSH
- Tongue chemistry ultrastructure MeSH
- Rats MeSH
- Microscopy, Electron, Scanning instrumentation methods MeSH
- Temperature MeSH
- Pressure MeSH
- Water chemistry MeSH
- Desiccation * instrumentation methods MeSH
- Environment MeSH
- Phase Transition MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Water MeSH
In this study a non-conductive biological sample is observed free of charging artefacts when placed on a cooled Peltier stage in the specimen chamber of an alternative form of the environmental scanning electron microscope, equipped with a specially designed hydration system. This system was used to create dynamically changing surrounding conditions leading to controlled dehydration of the sample enabling us to visualize the topographical structure of a rat tongue in the transition region between the liquid and the gas state of water in the microscope specimen chamber.
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