Temperature and pH affect the production of bacterial biofilm

. 2010 Jan ; 55 (1) : 75-8. [epub] 20100325

Jazyk angličtina Země Spojené státy americké Médium print-electronic

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid20336508

The effect of different cultivation temperatures (30 and 37 degrees C) and pH of the media (5.5, 7.5, 8.5) on the biofilm production was compared in Pseudomonas aeruginosa, Klebsiella pneumoniae, and Vibrio cholerae non-O1 and O1 using the crystal-violet test for estimation of quantitative production of the biofilm. Decrease (46.4-98.4 %) in the biofilm production was observed at 37 degrees C in 8 of the tested strains (P. aeruginosa three strains, K pneumoniae two, V. cholerae non-O1 two, and V. cholerae O1 one strain) compared with the production at 30 degrees C. On the other hand, five strains (P. aeruginosa 1, K. pneumoniae 3, V. cholerae non-O1 1) exhibited under these conditions a higher biofilm production (103-143 %). However, this difference was not significant (p = 0.196). Increased pH lead to a higher biofilm production using all media tested. In P. aeruginosa the biofilm production at pH 8.5 was 139-244 %, at pH 7.5 136-164 % in comparison with pH 5.5. Similarly, in K. pneumoniae the biofilm production increased to 151-319 % at pH 8.5 while with the drop of pH to 7.5 the biofilm production was 113-177 % compared with pH 5.5. In V. cholerae non-O1 and O1 the biofilm production reached 204-329 % at pH 8.5, and 123-316 % at pH 7.5 (compared with the production at pH 5.5). An increase in biofilm production represented an average of 169 % (p = 0.001) at pH change from 5.5 to 7.5, with the rise of pH from 5.5 to 8.5 caused an average difference of 229 % (p = 0.001).

Zobrazit více v PubMed

Annu Rev Microbiol. 1995;49:711-45 PubMed

Mol Microbiol. 1998 May;28(3):449-61 PubMed

Mol Gen Genet. 1987 Jul;208(3):511-7 PubMed

FEMS Microbiol Lett. 2000 Oct 1;191(1):31-6 PubMed

Lett Appl Microbiol. 2004;38(5):428-32 PubMed

Clin Infect Dis. 2001 Aug 1;33(3):317-23 PubMed

Nature. 2000 Oct 12;407(6805):762-4 PubMed

Science. 1999 May 21;284(5418):1318-22 PubMed

Clin Microbiol Rev. 1998 Oct;11(4):589-603 PubMed

Nat Rev Microbiol. 2004 Feb;2(2):95-108 PubMed

FEMS Immunol Med Microbiol. 1995 Oct;12(2):113-20 PubMed

Clin Microbiol Rev. 1995 Jan;8(1):48-86 PubMed

Folia Microbiol (Praha). 2005;50(2):99-102 PubMed

Can J Urol. 1997 Sep;4(3):416-421 PubMed

Epidemiol Mikrobiol Imunol. 2007 Nov;56(4):186-90 PubMed

Folia Microbiol (Praha). 2008;53(1):57-60 PubMed

Folia Microbiol (Praha). 2007;52(1):86-90 PubMed

Epidemiol Mikrobiol Imunol. 2004;53(2):66-9 PubMed

Najít záznam

Citační ukazatele

Nahrávání dat ...

Možnosti archivace

Nahrávání dat ...