The effect of arsenic contamination on amino acids metabolism in Spinacia oleracea L
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20655589
DOI
10.1016/j.ecoenv.2010.07.008
PII: S0147-6513(10)00158-2
Knihovny.cz E-resources
- MeSH
- Amino Acids metabolism MeSH
- Arsenic analysis pharmacokinetics toxicity MeSH
- Biomass MeSH
- Phosphotransferases (Carboxyl Group Acceptor) metabolism MeSH
- Soil Pollutants analysis pharmacokinetics toxicity MeSH
- Soil analysis MeSH
- Spectrophotometry, Atomic MeSH
- Spinacia oleracea drug effects enzymology growth & development metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Amino Acids MeSH
- Arsenic MeSH
- Phosphotransferases (Carboxyl Group Acceptor) MeSH
- glutamate 5-kinase MeSH Browser
- Soil Pollutants MeSH
- Soil MeSH
Changes of amino acid concentrations (proline, glutamate, asparagine, aspartate, alanine) and glutamate kinase activity (GKA) in plants under arsenic chronic stress reported here reveal their role in plant arsenic stress adaptation. Results of the pot experiment confirmed the toxic effect of arsenic at tested levels (As1=25 mg As kg(-1) soil, As2=50 mg As kg(-1) soil, As3=75 mg As kg(-1) soil) for spinach. Growing available arsenic contents in soil were associated with the strong inhibition of above-ground biomass and with the enhancement of As plant content. The changes of glutamate, asparagine, aspartate and proline levels in the plants showed strong linear dependences on arsenic concentration in plants (R2=0.60-0.90). Compared to the untreated control, concentrations of free proline and aspartate of As3 treatment were enhanced up to 381% and 162%, respectively. The significant changes of glutamate were observed on As2 and As3 treatments (increased level up to 188, i.e. 617%). Arsenic in plants was shown to be an inhibitor of glutamase kinase activity (R2=0.91). Inhibition of GKA resulted in an increase in the content of glutamate that is used in synthesis of phytochelatins in plant cells. Concentration of alanine did not have a confirmed linear dependence on arsenic concentration in plant (R2=0.05). The changes of its concentrations could be affected by changes of pH in plant cell or induction of alanine aminotransferase by hypoxia.
References provided by Crossref.org
Response of cytokinins and nitrogen metabolism in the fronds of Pteris sp. under arsenic stress
