The effect of arsenic contamination on amino acids metabolism in Spinacia oleracea L
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
20655589
DOI
10.1016/j.ecoenv.2010.07.008
PII: S0147-6513(10)00158-2
Knihovny.cz E-zdroje
- MeSH
- aminokyseliny metabolismus MeSH
- arsen analýza farmakokinetika toxicita MeSH
- biomasa MeSH
- fosfotransferasy s karboxylovou skupinou jako akceptorem metabolismus MeSH
- látky znečišťující půdu analýza farmakokinetika toxicita MeSH
- půda analýza MeSH
- spektrofotometrie atomová MeSH
- Spinacia oleracea účinky léků enzymologie růst a vývoj metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- aminokyseliny MeSH
- arsen MeSH
- fosfotransferasy s karboxylovou skupinou jako akceptorem MeSH
- glutamate 5-kinase MeSH Prohlížeč
- látky znečišťující půdu MeSH
- půda MeSH
Changes of amino acid concentrations (proline, glutamate, asparagine, aspartate, alanine) and glutamate kinase activity (GKA) in plants under arsenic chronic stress reported here reveal their role in plant arsenic stress adaptation. Results of the pot experiment confirmed the toxic effect of arsenic at tested levels (As1=25 mg As kg(-1) soil, As2=50 mg As kg(-1) soil, As3=75 mg As kg(-1) soil) for spinach. Growing available arsenic contents in soil were associated with the strong inhibition of above-ground biomass and with the enhancement of As plant content. The changes of glutamate, asparagine, aspartate and proline levels in the plants showed strong linear dependences on arsenic concentration in plants (R2=0.60-0.90). Compared to the untreated control, concentrations of free proline and aspartate of As3 treatment were enhanced up to 381% and 162%, respectively. The significant changes of glutamate were observed on As2 and As3 treatments (increased level up to 188, i.e. 617%). Arsenic in plants was shown to be an inhibitor of glutamase kinase activity (R2=0.91). Inhibition of GKA resulted in an increase in the content of glutamate that is used in synthesis of phytochelatins in plant cells. Concentration of alanine did not have a confirmed linear dependence on arsenic concentration in plant (R2=0.05). The changes of its concentrations could be affected by changes of pH in plant cell or induction of alanine aminotransferase by hypoxia.
Citace poskytuje Crossref.org
Response of cytokinins and nitrogen metabolism in the fronds of Pteris sp. under arsenic stress
