Stem cells from human exfoliated deciduous teeth--isolation, long term cultivation and phenotypical analysis
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Cell Differentiation * MeSH
- Antigens, CD analysis MeSH
- Child MeSH
- Adult MeSH
- Stem Cells cytology immunology MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Molar, Third cytology MeSH
- Cell Proliferation MeSH
- Tooth Exfoliation MeSH
- Dental Pulp cytology MeSH
- Tooth, Deciduous cytology MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, CD MeSH
AIMS: Our aims were to isolate stem cells from human exfoliated deciduous teeth (SHED), to cultivate them in vitro and to investigate their basic biological properties, phenotype and to compare our findings with dental pulp stem cells (DPSC) isolated from permanent teeth. METHODS: Dental pulp was gently evacuated from exfoliated teeth. After enzymatic dissociation of dental pulp, SHED were cultivated in modified cultivation media for mesenchymal adult progenitor cells containing 2% FCS and supplemented with growth factors and insulin, transferrin, sodium (ITS) supplement. Cell viability and other biological properties were examined using a Vi-Cell analyzer and a Z2-Counter. DNA analyses and phenotyping were performed with flow cytometry. RESULTS: We were able to cultivate SHED over 45 population doublings. Our results showed that SHED cultivated under same conditions as DPSC had longer average population doubling time (41.3 hrs for SHED vs. 24.5 hrs for DPSC). Phenotypic comparison of cultivated SHED to that of cultivated DPSC showed differential expression CD29, CD44, CD71, CD117, CD 166. During long-term cultivation, SHED did not showed any signs of degeneration or spontaneous differentiation. CONCLUSIONS: We isolated stem cells from exfoliated teeth. In comparison to DPSC, SHED proliferation rate was about 50% slower, and SHED showed slightly different phenotype. These cells may be extremely useful for stem cell tissue banking, further stem cell research and future therapeutic applications.
References provided by Crossref.org
Intra-Individual Variability of Human Dental Pulp Stem Cell Features Isolated from the Same Donor
The Effects of Cryogenic Storage on Human Dental Pulp Stem Cells
Taking a bite out of spinal cord injury: do dental stem cells have the teeth for it?
