Characterization of dental pulp stem cells from impacted third molars cultured in low serum-containing medium
Language English Country Switzerland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21071916
DOI
10.1159/000321160
PII: 000321160
Knihovny.cz E-resources
- MeSH
- Actins metabolism MeSH
- CD146 Antigen metabolism MeSH
- Cell Differentiation drug effects MeSH
- Cell Lineage drug effects MeSH
- Chondrogenesis drug effects MeSH
- Endothelial Cells cytology drug effects metabolism MeSH
- Immunohistochemistry MeSH
- Stem Cells drug effects pathology MeSH
- Culture Media, Serum-Free pharmacology MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Molar, Third pathology MeSH
- Myocytes, Smooth Muscle cytology drug effects metabolism MeSH
- Myofibroblasts cytology drug effects metabolism MeSH
- Neurons cytology drug effects metabolism MeSH
- Osteogenesis drug effects MeSH
- Flow Cytometry MeSH
- Tooth, Impacted pathology MeSH
- Dental Pulp pathology MeSH
- Check Tag
- Humans MeSH
- Adolescent MeSH
- Young Adult MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Actins MeSH
- CD146 Antigen MeSH
- Culture Media, Serum-Free MeSH
We isolated and expanded stem cells from dental pulp from extracted third molars using an innovative culture method consisting of low serum-containing medium supplemented with epidermal growth factor and platelet-derived growth factor BB. We evaluated the differentiation potential of these cells when they were growing either adherently or as micromass/spheroid cultures in various media. Undifferentiated and differentiated cells were analyzed by flow cytometry, immunocytochemistry and immunoblotting. The flow cytometry results showed that the dental pulp stem cells (DPSCs) were positive for mesenchymal stromal cell markers, but negative for hematopoietic markers. Immunocytochemical and/or immunoblotting analyses revealed the expression of numerous stem cell markers, including nanog, Sox2, nestin, Musashi-1 and nucleostemin, whereas they were negative for markers associated with differentiated neural, vascular and hepatic cells. Surprisingly, the cells were only slightly positive for α-smooth muscle actin, and a heterogeneous expression of CD146 was observed. When cultured in osteogenic media, they expressed osteonectin, osteopontin and procollagen I, and in micromass cultures, they produced collagen I. DPSCs cultured in TGF-β1/3-supplemented media produced extracellular matrix typical of cartilaginous tissue. The addition of vascular endothelial growth factor to serum-free media resulted in the expression of endothelial markers. Interestingly, when cultured in neurogenic media, DPSCs exhibited de novo or upregulated markers of undifferentiated and differentiated neural cells. Collectively, our data show that DPSCs are self-renewing and able to express markers of bone, cartilage, vascular and neural tissues, suggesting their multipotential capacity. Their easy accessibility makes these cells a suitable source of somatic stem cells for tissue engineering.
References provided by Crossref.org
The Effects of Cryogenic Storage on Human Dental Pulp Stem Cells
Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro
Telomere attrition occurs during ex vivo expansion of human dental pulp stem cells
