Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, oocyte maturation, and acquisition of developmental competence in vitro: comparison with gonadotropins
Language English Country England, Great Britain Media print-electronic
Document type Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
21239527
DOI
10.1530/rep-10-0418
PII: REP-10-0418
Knihovny.cz E-resources
- MeSH
- Cell Differentiation drug effects genetics MeSH
- Embryonic Development drug effects genetics MeSH
- Epidermal Growth Factor chemistry pharmacology MeSH
- Follicle Stimulating Hormone pharmacology MeSH
- Gonadotropins pharmacology MeSH
- Embryo Culture Techniques MeSH
- Cells, Cultured MeSH
- Cumulus Cells drug effects metabolism physiology MeSH
- Oocytes drug effects metabolism physiology MeSH
- Oogenesis drug effects genetics MeSH
- Parthenogenesis drug effects genetics physiology MeSH
- Peptide Fragments chemistry pharmacology MeSH
- Swine * genetics metabolism physiology MeSH
- Cell Proliferation drug effects MeSH
- Gene Expression Profiling MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Epidermal Growth Factor MeSH
- Follicle Stimulating Hormone MeSH
- Gonadotropins MeSH
- Peptide Fragments MeSH
The aim of this work was to assess the FSH-stimulated expression of epidermal growth factor (EGF)-like peptides in cultured cumulus-oocyte complexes (COCs) and to find out the effect of the peptides on cumulus expansion, oocyte maturation, and acquisition of developmental competence in vitro. FSH promptly stimulated expression of amphiregulin (AREG) and epiregulin (EREG), but not betacellulin (BTC) in the cultured COCs. Expression of AREG and EREG reached maximum at 2 or 4 h after FSH addition respectively. FSH also significantly stimulated expression of expansion-related genes (PTGS2, TNFAIP6, and HAS2) in the COCs at 4 and 8 h of culture, with a significant decrease at 20 h of culture. Both AREG and EREG also increased expression of the expansion-related genes; however, the relative abundance of mRNA for each gene was much lower than in the FSH-stimulated COCs. In contrast to FSH, AREG and EREG neither stimulated expression of CYP11A1 in the COCs nor an increase in progesterone production by cumulus cells. AREG and EREG stimulated maturation of oocytes and expansion of cumulus cells, although the percentage of oocytes that had reached metaphase II was significantly lower when compared to FSH-induced maturation. Nevertheless, significantly more oocytes stimulated with AREG and/or EREG developed to blastocyst stage after parthenogenetic activation when compared to oocytes stimulated with FSH alone or combinations of FSH/LH or pregnant mares serum gonadotrophin/human chorionic gonadotrophin. We conclude that EGF-like peptides do not mimic all effects of FSH on the cultured COCs; nevertheless, they yield oocytes with superior developmental competence.
References provided by Crossref.org
A Role of PI3K/Akt Signaling in Oocyte Maturation and Early Embryo Development
The Biological Role of Hyaluronan-Rich Oocyte-Cumulus Extracellular Matrix in Female Reproduction
Regulation of mitogen-activated protein kinase 3/1 activity during meiosis resumption in mammals
