Narrow-bore core-shell particles and monolithic columns in the analysis of silybin diastereoisomers
Language English Country Germany Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Chromolith, Core-shell, Kinetex, Monolithic column, Silybin,
- MeSH
- Silybin MeSH
- Silymarin chemistry isolation & purification MeSH
- Stereoisomerism MeSH
- Resins, Synthetic chemistry MeSH
- Chromatography, High Pressure Liquid instrumentation methods MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Silybin MeSH
- Silymarin MeSH
- Resins, Synthetic MeSH
Two chromatographic narrow-bore columns, a novel 2.6 μm particle-packed Kinetex™ C18 core-shell (50×2.1 mm id) and monolithic Chromolith(®) FastGradient RP-18e (50×2 mm id), were evaluated for the analysis of diastereoisomers of the flavonolignans silybin and 23-O-acetylsilybin under isocratic conditions. The main advantages of the core-shell column are markedly higher efficiency (hmin =2.8 versus 5.6 for silybin A) and better peak symmetry. The Kinetex column exhibits only a slight change in the height equivalent of the theoretical plate with a higher linear velocity of the mobile phase. The monolithic column shows notably higher selectivity in terms of selectivity factor (1.21 versus 1.12) in the analysis of critical-pair of diastereoisomers (silybin A and silybin B) and enables shorter run duration (approx. twofold) together with lower backpressure. The resolution power was found to be comparable, but the Kinetex column required a higher pressure of the mobile phase that, together with the higher chance of clogging, can be a disadvantage in the separation of biological samples. Successful baseline separation of silybin diastereoisomers in real pharmaceutical sample on monolithic column was accomplished.
References provided by Crossref.org
Chirality Matters: Biological Activity of Optically Pure Silybin and Its Congeners
