Silica-coated La0.75Sr0.25MnO3 nanoparticles for magnetically driven DNA isolation
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
21919199
DOI
10.1002/jssc.201100442
Knihovny.cz E-zdroje
- MeSH
- DNA bakterií chemie izolace a purifikace MeSH
- Lactobacillus cytologie genetika MeSH
- lanthan chemie MeSH
- magnetické jevy * MeSH
- magnetismus MeSH
- nanočástice chemie MeSH
- oxid křemičitý chemie MeSH
- oxidy chemie MeSH
- polymerázová řetězová reakce MeSH
- sloučeniny manganu chemie MeSH
- stroncium chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA bakterií MeSH
- lanthan MeSH
- manganese oxide MeSH Prohlížeč
- oxid křemičitý MeSH
- oxidy MeSH
- sloučeniny manganu MeSH
- stroncium MeSH
Magnetic La(0.75)Sr(0.25)MnO(3) nanoparticles possessing an approximately 20-nm-thick silica shell (LSMO(0.25)@SiO(2) ) were characterised and tested for the isolation of PCR-ready bacterial DNA. The results presented here show that the nanoparticles do not interfere in PCR. DNA was apparently reversibly adsorbed on their silica shell from the aqueous phase system (16% PEG 6000-2 M NaCl). The method proposed was used for DNA isolation from complex food samples (dairy products and probiotic food supplements). The isolated DNA was compatible with PCR. The main advantages of the nanoparticles tested for routine use were their high colloidal stability allowing a more precise dosage and therefore high reproducibility of DNA isolation.
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