CTX-M-15-producing Escherichia coli clone B2-O25b-ST131 and Klebsiella spp. isolates in municipal wastewater treatment plant effluents
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21954457
DOI
10.1093/jac/dkr363
PII: dkr363
Knihovny.cz E-resources
- MeSH
- Bacteriological Techniques methods MeSH
- beta-Lactamases metabolism MeSH
- Water Purification MeSH
- DNA, Bacterial genetics MeSH
- DNA Fingerprinting MeSH
- Escherichia coli classification enzymology genetics isolation & purification MeSH
- Virulence Factors genetics MeSH
- Genotype MeSH
- Klebsiella classification enzymology genetics isolation & purification MeSH
- Culture Media chemistry MeSH
- Humans MeSH
- Water Microbiology * MeSH
- Multilocus Sequence Typing MeSH
- Plasmids analysis MeSH
- Polymerase Chain Reaction MeSH
- Electrophoresis, Gel, Pulsed-Field MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- beta-lactamase CTX-M-15 MeSH Browser
- beta-Lactamases MeSH
- DNA, Bacterial MeSH
- Virulence Factors MeSH
- Culture Media MeSH
OBJECTIVES: The global occurrence of antibiotic resistance genes in bacteria in water environments is an increasing concern. Treated wastewater was sampled daily over a 45 day period from the outflow of a municipal wastewater treatment plant in Brno, Czech Republic, and examined for extended-spectrum β-lactamase (ESBL)-producing bacteria. METHODS: Water samples were cultivated on MacConkey agar with cefotaxime (2 mg/L) and individual colonies were examined for ESBL production. Phenotypic ESBL-positive bacteria identified as Escherichia coli or Klebsiella spp. were tested for the presence of antibiotic resistance genes, the virulence gene afa/dra and the bla(CTX-M) upstream region. Genetic relatedness was analysed by PFGE, multilocus sequence typing and plasmid analysis. RESULTS: A total of 68 ESBL-producing Enterobacteriaceae isolates were detected in 34 out of 45 wastewater samples. ESBL-producing isolates included 26 E. coli isolates, 4 Klebsiella pneumoniae isolates and 1 Klebsiella oxytoca isolate. The pandemic and multiresistant B2-O25b-ST131 clone was predominant, being detected among 19 E. coli isolates, and 17 of the B2-O25b-ST131 isolates were positive for the FIA replicon and the afa/dra operon and had an IS26 element flanking bla(CTX-M-15). Seventeen of the B2-O25b-ST131 isolates showed closely related PFGE profiles (defined by 84% band similarity) and belonged to identical clonal groups. CONCLUSIONS: The results highlight the inadequacy of the treatment process in removing multiresistant bacteria from municipal wastewater and point to a risk of transmission of clinically important multiresistant strains, such as the pandemic ST131 clone, to the environment. This is the first study demonstrating the pandemic ST131 clone in wastewater.
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