Male gametophyte development leading to the formation of a mature pollen grain is precisely controlled at various levels, including transcriptional, post-transcriptional and post-translational, during its whole progression. Transcriptomic studies exploiting genome-wide microarray technologies revealed the uniqueness of pollen transcriptome and the dynamics of early and late successive global gene expression programs. However, the knowledge of transcription regulation is still very limited. In this study, we focused on the identification of pollen-expressed transcription factor (TF) genes involved in the regulation of male gametophyte development. To achieve this, the reverse genetic approach was used. Seventy-four T-DNA insertion lines were screened, representing 49 genes of 21 TF families active in either early or late pollen development. In the screen, ten phenotype categories were distinguished, affecting various structural or functional aspects, including pollen abortion, presence of inclusions, variable pollen grain size, disrupted cell wall structure, cell cycle defects, and male germ unit organization. Thirteen lines were not confirmed to contain the T-DNA insertion. Among 61 confirmed lines, about half (29 lines) showed strong phenotypic changes (i.e., ≥ 25% aberrant pollen) including four lines that produced a remarkably high proportion (70-100%) of disturbed pollen. However, the remaining 32 lines exhibited mild defects or resembled wild-type appearance. There was no significant bias toward any phenotype category among early and late TF genes, nor, interestingly, within individual TF families. Presented results have a potential to serve as a basal information resource for future research on the importance of respective TFs in male gametophyte development.

Laboratory of Pollen Biology Institute of Experimental Botany ASCR Rozvojová 263 165 02 Prague 6 Czech Republic

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BMC Plant Biol. 2007 Jul 23;7:39 PubMed

Genome Biol. 2001;2(8):RESEARCH0032 PubMed

J Exp Bot. 2009;60(5):1479-92 PubMed

Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13679-84 PubMed

Plant Mol Biol. 2006 May;61(1-2):165-77 PubMed

Curr Opin Plant Biol. 2001 Oct;4(5):447-56 PubMed

Plant Cell. 2010 Mar;22(3):782-96 PubMed

Development. 1998 Oct;125(19):3789-99 PubMed

Plant Physiol. 2003 Jun;132(2):640-52 PubMed

Plant Cell. 2010 Jan;22(1):91-107 PubMed

Plant Cell. 2002 Dec;14(12):2985-94 PubMed

Curr Opin Plant Biol. 2001 Apr;4(2):111-7 PubMed

Plant Mol Biol. 2006 Jan;60(1):107-24 PubMed

Plant Physiol. 2006 Nov;142(3):1004-13 PubMed

Mol Plant. 2010 Mar;3(2):361-73 PubMed

Plant Physiol. 1998 Jul;117(3):1015-22 PubMed

Bioinformatics. 2005 May 15;21(10):2568-9 PubMed

J Proteome Res. 2009 Apr;8(4):2015-31 PubMed

Plant J. 2001 Oct;28(1):27-39 PubMed

Annu Rev Genet. 2002;36:99-124 PubMed

Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13579-84 PubMed

Plant J. 2004 Dec;40(6):979-95 PubMed

Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11664-9 PubMed

J Exp Bot. 2009;60(5):1465-78 PubMed

Plant Physiol. 2005 Jun;138(2):744-56 PubMed

Plant Cell. 2002 Oct;14(10):2353-67 PubMed

Planta. 2009 Feb;229(3):605-15 PubMed

Science. 1999 Dec 10;286(5447):2119-25 PubMed

EMBO J. 2005 May 18;24(10):1874-85 PubMed

Plant J. 2010 Mar;61(6):928-40 PubMed

Plant Mol Biol. 1999 Nov;41(5):577-85 PubMed

BMC Bioinformatics. 2003 Jun 23;4:25 PubMed

Plant Physiol. 2009 Apr;149(4):1713-23 PubMed

Plant Physiol. 2004 Apr;134(4):1586-97 PubMed

Proc Natl Acad Sci U S A. 1999 Sep 28;96(20):11416-21 PubMed

Plant Cell. 1998 Jun;10(6):1043-54 PubMed

Plant Cell Physiol. 2008 Oct;49(10):1429-50 PubMed

PLoS Genet. 2009 Mar;5(3):e1000440 PubMed

J Exp Bot. 2006;57(11):2709-17 PubMed

Plant Physiol. 2007 Sep;145(1):98-105 PubMed

Plant Cell Physiol. 2002 Nov;43(11):1285-92 PubMed

Development. 1996 Oct;122(10):3243-53 PubMed

Plant Mol Biol. 2009 Jan;69(1-2):107-19 PubMed

Science. 2003 Aug 1;301(5633):653-7 PubMed

Plant J. 2003 Jan;33(2):413-23 PubMed

Plant Cell. 2007 Nov;19(11):3530-48 PubMed

Science. 1990 Oct 12;250(4978):267-71 PubMed

Genetics. 2004 Aug;167(4):1975-86 PubMed

Planta. 2008 Oct;228(5):709-23 PubMed

Plant Cell. 1990 Aug;2(8):755-67 PubMed

Gene. 2010 Jun 1;457(1-2):1-12 PubMed

Plant Physiol. 2004 Sep;136(1):2734-46 PubMed

Adv Exp Med Biol. 2011;722:118-36 PubMed

Prog Nucleic Acid Res Mol Biol. 1993;46:51-91 PubMed

Sex Plant Reprod. 2004 May 26;17(2):63-70 PubMed

Plant Physiol. 2005 Jan;137(1):297-307 PubMed

Genes Dev. 2002 Aug 1;16(15):2021-31 PubMed

Curr Opin Biotechnol. 2000 Apr;11(2):157-61 PubMed

Bioinformatics. 2009 Jan 15;25(2):159-62 PubMed

Plant Cell. 2000 Jul;12(7):1093-101 PubMed

Development. 2006 Aug;133(16):3085-95 PubMed

Plant Physiol. 2003 Apr;131(4):1855-67 PubMed

Plant Cell. 1998 Jun;10(6):925-36 PubMed

Genetics. 1998 Jun;149(2):621-31 PubMed

BMC Genomics. 2008 Jan 27;9:44 PubMed

Curr Biol. 2002 Oct 15;12(20):1718-27 PubMed

Plant Mol Biol. 2009 Jul;70(5):581-601 PubMed

J Integr Plant Biol. 2008 Jul;50(7):886-96 PubMed

PLoS Genet. 2009 Mar;5(3):e1000430 PubMed

Gene. 2006 Feb 1;366(2):256-65 PubMed

Plant Physiol. 2008 Oct;148(2):1168-81 PubMed

Plant Physiol. 2008 Nov;148(3):1201-11 PubMed

Genes Dev. 1999 Aug 15;13(16):2108-17 PubMed

Plant Physiol. 2004 Sep;136(1):2621-32 PubMed

Plant Physiol. 2006 Mar;140(3):818-29 PubMed

Genome Biol. 2007;8(11):R249 PubMed

Plant Physiol. 2002 Jun;129(2):865-75 PubMed

Plant Cell. 1996 Jul;8(7):1107-19 PubMed

Plant Cell. 2006 Nov;18(11):2971-84 PubMed

Genome Res. 2009 Oct;19(10):1786-800 PubMed

Nature. 2007 Nov 1;450(7166):119-23 PubMed

Plant Cell. 2005 Mar;17(3):705-21 PubMed

Biol Chem. 1998 Jun;379(6):633-46 PubMed

J Exp Bot. 2010 Sep;61(14):3901-14 PubMed

Sex Plant Reprod. 2011 Jun;24(2):149-60 PubMed

Development. 2006 May;133(9):1645-55 PubMed

Plant J. 2009 Nov;60(3):476-87 PubMed

Plant Cell. 2010 Mar;22(3):672-89 PubMed

Plant J. 2010 Jul 1;63(1):155-66 PubMed

PLoS Genet. 2009 Aug;5(8):e1000621 PubMed

Plant Cell. 2005 Aug;17(8):2384-96 PubMed

Mol Biol Evol. 2007 Mar;24(3):670-8 PubMed

Cell Biol Int. 2003;27(7):559-66 PubMed

Plant J. 2003 May;34(4):519-28 PubMed

Plant Mol Biol. 2006 May;61(1-2):13-30 PubMed

J Plant Physiol. 2010 Nov 15;167(17):1512-20 PubMed

Gene. 2007 Jun 1;394(1-2):13-24 PubMed

Plant Cell. 2007 Nov;19(11):3549-62 PubMed

Plant Cell. 2005 May;17(5):1424-33 PubMed

Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):31-6 PubMed

Gene. 2000 May 2;248(1-2):23-32 PubMed

Plant Cell. 2009 Apr;21(4):1109-28 PubMed

Plant Cell. 2004 Oct;16(10):2601-13 PubMed

J Biochem Mol Biol. 2007 Nov 30;40(6):1083-9 PubMed

Genome Biol. 2004;5(11):R85 PubMed

Plant J. 1998 Jul;15(1):79-88 PubMed

Plant Cell. 2001 May;13(5):1231-44 PubMed

Plant J. 2000 Nov;24(4):457-66 PubMed

Plant Physiol. 2009 Nov;151(3):1486-97 PubMed

Plant Mol Biol. 2006 May;61(1-2):215-26 PubMed

Plant Cell. 2008 Jul;20(7):1760-74 PubMed

Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):2152-6 PubMed

Development. 2005 Feb;132(3):603-14 PubMed

J Biol Chem. 2002 Jul 19;277(29):26429-35 PubMed

Plant Cell. 2003 Jan;15(1):63-78 PubMed

Trends Plant Sci. 2002 Mar;7(3):106-11 PubMed

Genetics. 1997 Nov;147(3):1411-22 PubMed

Plant Mol Biol. 2006 Jun;61(3):415-30 PubMed

Science. 2000 Dec 15;290(5499):2105-10 PubMed

Nature. 2004 Jul 15;430(6997):356-60 PubMed

Plant Physiol. 2006 Mar;140(3):890-8 PubMed

Plant Cell. 2003 Aug;15(8):1749-70 PubMed

Mol Gen Genet. 1998 Dec;260(5):444-52 PubMed

Mol Cells. 2009 Apr 30;27(4):481-90 PubMed

Genetics. 1999 Feb;151(2):849-63 PubMed

Genetics. 2004 Oct;168(2):971-82 PubMed

Plant Physiol. 2003 Oct;133(2):713-25 PubMed

Plant J. 2007 Nov;52(3):528-38 PubMed

Plant J. 2004 Apr;38(2):332-47 PubMed

Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W465-70 PubMed

Dev Biol. 2005 Sep 1;285(1):91-100 PubMed

Plant J. 2003 Jul;35(2):177-92 PubMed

Curr Biol. 2005 Feb 8;15(3):244-8 PubMed

Cell. 1997 Oct 17;91(2):149-51 PubMed

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