Sexual plant reproduction depends on the production and differentiation of functional gametes by the haploid gametophyte generation. Currently, we have a limited understanding of the regulatory mechanisms that have evolved to specify the gametophytic developmental programs. To unravel such mechanisms, it is necessary to identify transcription factors (TF) that are part of such haploid regulatory networks. Here we focus on bZIP TFs that have critical roles in plants, animals and other kingdoms. We report the functional characterization of Arabidopsis thaliana AtbZIP34 that is expressed in both gametophytic and surrounding sporophytic tissues during flower development. T-DNA insertion mutants in AtbZIP34 show pollen morphological defects that result in reduced pollen germination efficiency and slower pollen tube growth both in vitro and in vivo. Light and fluorescence microscopy revealed misshapen and misplaced nuclei with large lipid inclusions in the cytoplasm of atbzip34 pollen. Scanning and transmission electron microscopy revealed defects in exine shape and micropatterning and a reduced endomembrane system. Several lines of evidence, including the AtbZIP34 expression pattern and the phenotypic defects observed, suggest a complex role in male reproductive development that involves a sporophytic role in exine patterning, and a sporophytic and/or gametophytic mode of action of AtbZIP34 in several metabolic pathways, namely regulation of lipid metabolism and/or cellular transport.

Laboratory of Pollen Biology Institute of Experimental Botany ASCR Rozvojová 263 Prague 6 Czech Republic

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BMC Plant Biol. 2007 Jul 23;7:39 PubMed

Trends Plant Sci. 2005 Sep;10(9):407-9 PubMed

Trends Plant Sci. 2005 Mar;10(3):103-5 PubMed

Nature. 1992 Dec 24-31;360(6406):741-5 PubMed

Plant Cell. 2004;16 Suppl:S142-53 PubMed

Plant J. 2007 Nov;52(3):570-82 PubMed

Plant Mol Biol. 2006 May;61(1-2):165-77 PubMed

J Biol Chem. 1998 Nov 13;273(46):30057-60 PubMed

Development. 1998 Oct;125(19):3789-99 PubMed

Plant Physiol. 2003 Jun;132(2):640-52 PubMed

Plant J. 2004 Jul;39(2):170-81 PubMed

Plant J. 2008 Jul;55(2):266-77 PubMed

Proc Natl Acad Sci U S A. 2007 Oct 9;104(41):16371-6 PubMed

Biochimie. 1993;75(3-4):225-30 PubMed

Plant Cell. 2000 Apr;12(4):599-609 PubMed

Plant Cell Physiol. 2003 Nov;44(11):1192-201 PubMed

Plant J. 1998 Dec;16(6):735-43 PubMed

Trends Plant Sci. 2008 Oct;13(10):550-6 PubMed

Plant Cell. 2007 Dec;19(12):4111-9 PubMed

J Biol Chem. 2000 Jan 21;275(3):1723-30 PubMed

EMBO J. 1992 Apr;11(4):1261-73 PubMed

Science. 1997 Dec 19;278(5346):2123-6 PubMed

Biochem Biophys Res Commun. 2008 Sep 19;374(2):242-7 PubMed

Plant Physiol. 2008 Dec;148(4):1938-52 PubMed

Annu Rev Biochem. 1991;60:73-99 PubMed

Plant Mol Biol. 2003 Sep;53(1-2):107-16 PubMed

J Exp Bot. 2009;60(5):1465-78 PubMed

Plant Physiol. 2005 Jun;138(2):744-56 PubMed

Planta. 2009 Feb;229(3):605-15 PubMed

Biochem Biophys Res Commun. 2007 Oct 19;362(2):425-30 PubMed

Plant Physiol. 2004 Sep;136(1):2532-47 PubMed

Plant Cell. 1995 Jan;7(1):65-74 PubMed

Plant Cell. 2000 Jun;12(6):901-15 PubMed

Plant J. 2004 Mar;37(6):914-39 PubMed

PLoS One. 2008 Aug 13;3(8):e2944 PubMed

Plant Cell Physiol. 2008 Jan;49(1):58-67 PubMed

Plant Physiol. 2008 Jun;147(2):852-63 PubMed

Trends Plant Sci. 2008 Apr;13(4):151-9 PubMed

J Biol Chem. 2001 Aug 10;276(32):30231-44 PubMed

J Exp Bot. 2006;57(11):2709-17 PubMed

Plant Physiol. 2008 Feb;146(2):333-50 PubMed

Plant Mol Biol. 2009 Jan;69(1-2):107-19 PubMed

Plant Cell. 2007 Nov;19(11):3530-48 PubMed

Mol Cell Biol. 2000 Jul;20(13):4773-81 PubMed

Plant Cell. 1990 Aug;2(8):755-67 PubMed

Mol Cell Biol. 1996 Feb;16(2):704-11 PubMed

Plant Physiol. 2005 Jan;137(1):297-307 PubMed

Curr Opin Plant Biol. 2008 Dec;11(6):695-701 PubMed

Plant J. 2007 May;50(3):381-90 PubMed

Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11632-7 PubMed

J Cell Biol. 2005 Jan 31;168(3):389-99 PubMed

BMC Plant Biol. 2005 Oct 07;5:22 PubMed

Mol Biol Evol. 2006 Aug;23(8):1480-92 PubMed

Genome Biol. 2007;8(11):R249 PubMed

Trends Plant Sci. 2001 Aug;6(8):347-8 PubMed

Zygote. 1993 May;1(2):173-9 PubMed

Genome Biol. 2001;2(8):RESEARCH0032 PubMed

Nature. 2002 Apr 25;416(6883):870-4 PubMed

Plant J. 2007 Sep;51(5):897-909 PubMed

Plant Physiol. 2001 Dec;127(4):1739-49 PubMed

Curr Opin Genet Dev. 1995 Oct;5(5):565-70 PubMed

Plant Cell. 2005 Mar;17(3):705-21 PubMed

EMBO J. 1995 Dec 15;14(24):6193-208 PubMed

J Cell Biol. 1999 May 31;145(5):1049-61 PubMed

Biochim Biophys Acta. 2000 Dec 29;1543(2):223-238 PubMed

Plant J. 2006 Jun;46(5):890-900 PubMed

Plant Physiol. 2007 Jan;143(1):447-60 PubMed

Plant Cell. 2007 Nov;19(11):3549-62 PubMed

Plant Cell. 2008 Nov;20(11):3107-21 PubMed

Proc Natl Acad Sci U S A. 2001 Jan 2;98(1):31-6 PubMed

Genome Biol. 2004;5(11):R85 PubMed

BMB Rep. 2008 Feb 29;41(2):132-8 PubMed

Biochim Biophys Acta. 1991 Feb 26;1082(1):1-26 PubMed

Trends Plant Sci. 2002 Mar;7(3):106-11 PubMed

Plant J. 2005 May;42(3):315-28 PubMed

Proc Natl Acad Sci U S A. 2005 Apr 5;102(14):5280-5 PubMed

Science. 2003 Jun 27;300(5628):2097-101 PubMed

Prog Lipid Res. 2001 Sep;40(5):325-438 PubMed

J Plant Res. 2008 Nov;121(6):603-11 PubMed

EMBO J. 1997 Sep 1;16(17):5247-59 PubMed

Plant J. 1997 Sep;12(3):615-23 PubMed

Proc Soc Exp Biol Med. 1995 Mar;208(3):263-70 PubMed

Plant Physiol. 2006 Apr;140(4):1151-68 PubMed

Plant J. 2007 Nov;52(3):528-38 PubMed

Planta. 2002 Jan;214(3):345-55 PubMed

Plant J. 2004 Apr;38(2):332-47 PubMed

Plant Cell. 2008 Feb;20(2):438-51 PubMed

Curr Biol. 2005 Feb 8;15(3):244-8 PubMed

J Biol Chem. 1990 Nov 15;265(32):19486-94 PubMed

Plant Mol Biol. 1998 Aug;37(6):977-88 PubMed

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