The slower the better: how sperm capacitation and acrosome reaction is modified in the presence of estrogens
Language English Country Great Britain, England Media print-electronic
Document type Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
22143972
DOI
10.1530/rep-11-0326
PII: REP-11-0326
Knihovny.cz E-resources
- MeSH
- Acrosome Reaction drug effects physiology MeSH
- Semen Analysis MeSH
- Time Factors MeSH
- Estrogens pharmacology MeSH
- Phosphorylation drug effects MeSH
- Sperm Capacitation drug effects physiology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Osmolar Concentration MeSH
- Protein-Tyrosine Kinases metabolism MeSH
- Dose-Response Relationship, Drug MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Estrogens MeSH
- Protein-Tyrosine Kinases MeSH
In order for mammalian sperm to obtain a fertilizing ability, they must undergo a complex of molecular changes, called capacitation. During capacitation, steroidal compounds can exert a fast nongenomic response in sperm through their interaction with plasma membrane receptors, and activate crucial signaling pathways leading to time-dependent protein tyrosine phosphorylation (TyrP). Estrogen receptor beta was detected in epididymal mouse sperm; therefore, the effect of 17B-estradiol, estrone, estriol, and 17A-ethynylestradiol on mouse sperm capacitation in vitro was investigated. The effect was evaluated by positive TyrP in sperm heads and in the whole sperm lysates. Simultaneously, the state of the acrosome after the calcium ionophore-induced acrosome reaction was assessed. Generally, estrogens displayed a time and concentration-dependent stimulatory effect on sperm TyrP during capacitation. In contrast, the number of sperm that underwent the acrosome reaction was lower in the experimental groups. It has been demonstrated that both natural and synthetic estrogens can modify the physiological progress of mouse sperm capacitation. The potential risk in the procapacitation effect of estrogens can also be seen in the decreased ability of sperm to undergo the acrosome reaction. In conclusion, the capacitating ability of sperm can be significantly lowered by increasing the level of estrogens in the environment.
References provided by Crossref.org
17α-Ethynylestradiol alters testicular epigenetic profiles and histone-to-protamine exchange in mice
αV Integrin Expression and Localization in Male Germ Cells
New Insight into Sperm Capacitation: A Novel Mechanism of 17β-Estradiol Signalling
CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization
