Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22301891
DOI
10.3732/ajb.1100416
PII: ajb.1100416
Knihovny.cz E-resources
- MeSH
- Asteraceae classification genetics MeSH
- Cell Nucleus genetics MeSH
- Chromosomes, Plant genetics MeSH
- Diploidy MeSH
- DNA Primers genetics MeSH
- DNA, Plant genetics MeSH
- Phylogeny MeSH
- Genetic Variation MeSH
- Genetic Markers MeSH
- Gene Dosage MeSH
- Gene Library MeSH
- DNA, Intergenic genetics MeSH
- Pseudogenes MeSH
- Genes, Plant * MeSH
- Base Sequence MeSH
- Sequence Analysis, DNA MeSH
- Computational Biology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA Primers MeSH
- DNA, Plant MeSH
- Genetic Markers MeSH
- DNA, Intergenic MeSH
PREMISE OF THE STUDY: The development of three low-copy nuclear markers for low taxonomic level phylogenies in Asteraceae with emphasis on the subtribe Hieraciinae is reported. METHODS AND RESULTS: Marker candidates were selected by comparing a Lactuca complementary DNA (cDNA) library with public DNA sequence databases. Interspecific variation and phylogenetic signal of the selected genes were investigated for diploid taxa from the subtribe Hieraciinae and compared to a reference phylogeny. Their ability to cross-amplify was assessed for other Asteraceae tribes. All three markers had higher variation (2.1-4.5 times) than the internal transcribed spacer (ITS) in Hieraciinae. Cross-amplification was successful in at least seven other tribes of the Asteraceae. Only three cases indicating the presence of paralogs or pseudogenes were detected. CONCLUSIONS: The results demonstrate the potential of these markers for phylogeny reconstruction in the Hieraciinae as well as in other Asteraceae tribes, especially for very closely related species.
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