Tumor promoting effects of cyanobacterial extracts are potentiated by anthropogenic contaminants--evidence from in vitro study
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22572165
DOI
10.1016/j.chemosphere.2012.04.008
PII: S0045-6535(12)00495-X
Knihovny.cz E-resources
- MeSH
- Alkaloids MeSH
- Aphanizomenon metabolism MeSH
- Bacterial Toxins MeSH
- Cell Extracts toxicity MeSH
- Cell Line MeSH
- Cylindrospermopsis metabolism MeSH
- Epithelial Cells drug effects metabolism MeSH
- Fluorenes toxicity MeSH
- Carcinogens toxicity MeSH
- Rats MeSH
- Environmental Pollutants toxicity MeSH
- Gap Junctions drug effects metabolism MeSH
- Cell Communication drug effects physiology MeSH
- Microcystins toxicity MeSH
- Marine Toxins MeSH
- Polychlorinated Biphenyls toxicity MeSH
- Drug Synergism MeSH
- Cyanobacteria Toxins MeSH
- Uracil analogs & derivatives toxicity MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 2,4,5,2',4',5'-hexachlorobiphenyl MeSH Browser
- Alkaloids MeSH
- Bacterial Toxins MeSH
- Cell Extracts MeSH
- cyanoginosin LR MeSH Browser
- cylindrospermopsin MeSH Browser
- fluoranthene MeSH Browser
- Fluorenes MeSH
- Carcinogens MeSH
- Environmental Pollutants MeSH
- Microcystins MeSH
- Marine Toxins MeSH
- Polychlorinated Biphenyls MeSH
- Cyanobacteria Toxins MeSH
- Uracil MeSH
Inhibition of gap junctional intercellular communication (GJIC) is affiliated with tumor promotion process and it has been employed as an in vitro biomarker for evaluation of tumor promoting effects of chemicals. In the present study we investigated combined effects of anthropogenic environmental contaminants 2,2',4,4',5,5'-hexachlorobiphenyl (PCB 153) and fluoranthene, cyanotoxins microcystin-LR and cylindrospermopsin, and extracts of laboratory cultures of cyanobacteria Aphanizomenon gracile and Cylindrospermopsis raciborskii, on GJIC in the rat liver epithelial cell line WB-F344. Binary mixtures of PCB 153 with fluoranthene and the mixtures of the two cyanobacterial strains elicited simple additive effects on GJIC after 30 min exposure, whereas microcystin-LR and cylindrospermopsin neither inhibited GJIC nor altered effects of PCB 153 or fluoranthene. However, synergistic effects were observed in the cells exposed to binary mixtures of anthropogenic contaminants (PCB 153 or fluoranthene) and cyanobacterial extracts. The synergistic effects were especially pronounced after prolonged (6-24h) co-exposure to fluoranthene and A. gracile extract, when mixture caused nearly complete GJIC inhibition, while none of the individual components caused any downregulation of GJIC at the same concentration and exposure time. The effects of cyanobacterial extracts were independent of microcystin-LR or cylindrospermopsin, which were not detected in cyanobacterial biomass. It provides further evidence on the presence of unknown tumor promoting metabolites in cyanobacteria. Clear potentiation of the GJIC inhibition observed in the mixtures of two anthropogenic contaminants and cyanobacteria highlight the importance of combined toxic effects of chemicals in complex environmental mixtures.
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