Tumor promoting effects of cyanobacterial extracts are potentiated by anthropogenic contaminants--evidence from in vitro study
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
22572165
DOI
10.1016/j.chemosphere.2012.04.008
PII: S0045-6535(12)00495-X
Knihovny.cz E-zdroje
- MeSH
- alkaloidy MeSH
- Aphanizomenon metabolismus MeSH
- bakteriální toxiny MeSH
- buněčné extrakty toxicita MeSH
- buněčné linie MeSH
- Cylindrospermopsis metabolismus MeSH
- epitelové buňky účinky léků metabolismus MeSH
- fluoreny toxicita MeSH
- karcinogeny toxicita MeSH
- krysa rodu Rattus MeSH
- látky znečišťující životní prostředí toxicita MeSH
- mezerový spoj účinky léků metabolismus MeSH
- mezibuněčná komunikace účinky léků fyziologie MeSH
- mikrocystiny toxicita MeSH
- mořské toxiny MeSH
- polychlorované bifenyly toxicita MeSH
- synergismus léků MeSH
- toxiny kmene Cyanobacteria MeSH
- uracil analogy a deriváty toxicita MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 2,4,5,2',4',5'-hexachlorobiphenyl MeSH Prohlížeč
- alkaloidy MeSH
- bakteriální toxiny MeSH
- buněčné extrakty MeSH
- cyanoginosin LR MeSH Prohlížeč
- cylindrospermopsin MeSH Prohlížeč
- fluoranthene MeSH Prohlížeč
- fluoreny MeSH
- karcinogeny MeSH
- látky znečišťující životní prostředí MeSH
- mikrocystiny MeSH
- mořské toxiny MeSH
- polychlorované bifenyly MeSH
- toxiny kmene Cyanobacteria MeSH
- uracil MeSH
Inhibition of gap junctional intercellular communication (GJIC) is affiliated with tumor promotion process and it has been employed as an in vitro biomarker for evaluation of tumor promoting effects of chemicals. In the present study we investigated combined effects of anthropogenic environmental contaminants 2,2',4,4',5,5'-hexachlorobiphenyl (PCB 153) and fluoranthene, cyanotoxins microcystin-LR and cylindrospermopsin, and extracts of laboratory cultures of cyanobacteria Aphanizomenon gracile and Cylindrospermopsis raciborskii, on GJIC in the rat liver epithelial cell line WB-F344. Binary mixtures of PCB 153 with fluoranthene and the mixtures of the two cyanobacterial strains elicited simple additive effects on GJIC after 30 min exposure, whereas microcystin-LR and cylindrospermopsin neither inhibited GJIC nor altered effects of PCB 153 or fluoranthene. However, synergistic effects were observed in the cells exposed to binary mixtures of anthropogenic contaminants (PCB 153 or fluoranthene) and cyanobacterial extracts. The synergistic effects were especially pronounced after prolonged (6-24h) co-exposure to fluoranthene and A. gracile extract, when mixture caused nearly complete GJIC inhibition, while none of the individual components caused any downregulation of GJIC at the same concentration and exposure time. The effects of cyanobacterial extracts were independent of microcystin-LR or cylindrospermopsin, which were not detected in cyanobacterial biomass. It provides further evidence on the presence of unknown tumor promoting metabolites in cyanobacteria. Clear potentiation of the GJIC inhibition observed in the mixtures of two anthropogenic contaminants and cyanobacteria highlight the importance of combined toxic effects of chemicals in complex environmental mixtures.
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