IGF2BP2 and IGF2 genetic effects in diabetes and diabetic nephropathy
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
22770937
DOI
10.1016/j.jdiacomp.2012.05.012
PII: S1056-8727(12)00166-3
Knihovny.cz E-zdroje
- MeSH
- diabetes mellitus 1. typu komplikace genetika metabolismus MeSH
- diabetes mellitus 2. typu komplikace genetika metabolismus MeSH
- diabetické nefropatie komplikace genetika metabolismus MeSH
- genetické asociační studie MeSH
- insulinu podobný růstový faktor II genetika metabolismus MeSH
- jednonukleotidový polymorfismus * MeSH
- kohortové studie MeSH
- ledviny růst a vývoj metabolismus MeSH
- lidé MeSH
- mutantní kmeny myší MeSH
- myši MeSH
- pohlavní dimorfismus MeSH
- proteiny vázající RNA genetika metabolismus MeSH
- regulace genové exprese MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Spojené státy americké MeSH
- Švédsko MeSH
- Názvy látek
- IGF2 protein, human MeSH Prohlížeč
- IGF2 protein, mouse MeSH Prohlížeč
- IGF2BP2 protein, human MeSH Prohlížeč
- IGF2BP2 protein, mouse MeSH Prohlížeč
- insulinu podobný růstový faktor II MeSH
- proteiny vázající RNA MeSH
OBJECTIVE: The IGF2BP2 gene is located on chromosome 3q27.2 within a region linked to type 1 diabetes (T1D), type 2 diabetes (T2D) and diabetic nephropathy (DN). Its protein functionally binds to 5'-UTR of the imprinting IGF2 gene. The present study aims to evaluate the IGF2BP2-IGF2 genetic effects in diabetes and DN. MATERIALS AND METHODS: Three cohorts including T1D with and without DN (n=1139) of European descents from the GoKinD study, Swedish T1D with and without DN (n=303) and Czech control subjects without diabetes, T1D, T2D with and without DN (n=1418) were enrolled in TaqMan genotyping experiments for IGF2BP2 rs4402960 and IGF2 rs10770125. Igf2bp2 gene expression in kidney tissues of db/db and control mice at the ages of 5 and 26 weeks was examined with real time RT-PCR and Western blot. RESULTS: An association of IGF2BP2 rs4402960 with T2D in the Czech population was replicated. This IGF2BP2 polymorphism (P=0.037, OR=0.69 95% CI 0.49-0.98) was found to be associated with DN in male not in female patients with T1D selected from the GoKinD study. In the analyses of combined the GoKinD, Czech and Swedish populations, the association between IGF2BP2 polymorphism and DN in male patients with T1D was still significant (P=0.030, OR=0.73, 95% CI 0.54-0.97). IGF2 rs10770125 was also associated with DN in male T1D patients of the GoKinD population (P=0.038, OR=0.67 95% CI 0.46-0.98). There might be a genetic interaction between IGF2BP2 and IGF2 (P=0.05). The Igf2bp2 gene expression levels were increased in the kidneys of db/db mice compared to controls at the age of 5weeks but not at 26 weeks. CONCLUSIONS: The present study has replicated the association of IGF2BP2 rs4402960 with T2D in the Czech population and provided data suggesting that IGF2BP2 may have genetic interaction with IGF2 with a protective effect against DN in male patients with T1D.
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