Real-time label-free monitoring of the cellular response to osmotic stress using conventional and long-range surface plasmons
Language English Country England, Great Britain Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22863117
DOI
10.1016/j.bios.2012.07.020
PII: S0956-5663(12)00460-5
Knihovny.cz E-resources
- MeSH
- Equipment Failure Analysis MeSH
- Staining and Labeling MeSH
- Biosensing Techniques instrumentation MeSH
- Cell Line MeSH
- Equipment Design MeSH
- Rats MeSH
- Kidney cytology physiology MeSH
- Osmotic Pressure MeSH
- Computer Systems MeSH
- Surface Plasmon Resonance instrumentation MeSH
- Reproducibility of Results MeSH
- Sensitivity and Specificity MeSH
- Cell Size MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Cell volume and its regulation are key factors for cellular integrity and also serve as indicators of various cell pathologies. SPR sensors represent an efficient tool for real-time and label-free observations of changes in cell volume and shape. Here, we extend this concept by employing the use of long-range surface plasmons (LRSP). Due to the enhanced penetration depth of LRSP (~1μm, compared to ~0.4μm of a conventional surface plasmon), the observation of refractive index changes occurring deeper inside the cells is possible. In this work, the responses of a confluent normal rat kidney (NRK) epithelial cell layer to osmotic stress are studied by both conventional and long-range surface plasmons. Experiments are conducted in parallel using cell layers grown and stimulated under the same conditions to enable direct comparison of the results and discrimination of the osmotic stress-induced effects in different parts of the cell.
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