Male-specific repeats in wild Bovidae
Jazyk angličtina Země Anglie, Velká Británie Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- chromozom Y chemie genetika MeSH
- druhová specificita MeSH
- fluorescenční barviva chemie MeSH
- fylogeneze MeSH
- hybridizace in situ fluorescenční veterinární MeSH
- laserová záchytná mikrodisekce metody veterinární MeSH
- metafáze MeSH
- molekulární evoluce MeSH
- populační genetika metody MeSH
- repetitivní sekvence nukleových kyselin * MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA metody veterinární MeSH
- sekvenční seřazení MeSH
- sexuální faktory MeSH
- skot klasifikace genetika MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- skot klasifikace genetika MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fluorescenční barviva MeSH
In this study, we investigated repetitive sequences localized on Y chromosomes. Repetitive DNA sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. Efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for Y chromosome separation, followed by polymerase chain reaction (PCR), cloning, and sequence analysis. We applied the derived repeat units to members of nine tribes within the Bovidae. Apart from the Y chromosomes of Bos taurus and Bubalus bubalis, where we used known sequences of repetition, the derived sequences were used as probes for fluorescent in situ cross-hybridization to members of the nine tribes of the Bovidae. We investigated the distribution of repeat units within the tribes and their localization on the Y chromosome. Sharing of sequence variants would indicate common descent, while the rapid horizontal evolution should allow discrimination between closely related species or subspecies.
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X Chromosome-Specific Repeats in Non-Domestic Bovidae
Meiotic behaviour of evolutionary sex-autosome translocations in Bovidae
Tribe-specific satellite DNA in non-domestic Bovidae
