The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu srovnávací studie, hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
12498346
DOI
10.1023/a:1020914702767
Knihovny.cz E-zdroje
- MeSH
- chromozomy genetika MeSH
- disekce metody MeSH
- DNA sondy * MeSH
- druhová specificita MeSH
- Equidae MeSH
- genetické markery MeSH
- heterozygot MeSH
- hospodářská zvířata genetika MeSH
- hybridizace in situ fluorescenční MeSH
- karyotypizace MeSH
- lasery * MeSH
- malování chromozomů metody MeSH
- prasata MeSH
- pruhování chromozomů MeSH
- skot MeSH
- Taq-polymerasa genetika MeSH
- translokace genetická MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- DNA sondy * MeSH
- genetické markery MeSH
- Taq-polymerasa MeSH
Laser microbeam microdissection and laser pressure catapulting procedure were used for the construction of chromosome-specific painting probes, arm-specific probes and probes for chromosomal subfragments. We report on a method for generation of fluorescence in-situ hybridization probes from laser dissected chromosomes of farm animals. So far, using the described method, a set of chromosome-specific painting probes has been obtained for all porcine chromosomes, 17 chromosomes of cattle and selected equine chromosomes. It is concluded that the laser technology appears to be a useful and powerful tool for the construction of chromosome-specifi c painting probes. Its main advantage is the fast non-contact collection of chromosomes.
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