The exocyst complex contributes to PIN auxin efflux carrier recycling and polar auxin transport in Arabidopsis
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
23163883
DOI
10.1111/tpj.12074
Knihovny.cz E-resources
- MeSH
- Arabidopsis cytology drug effects genetics metabolism MeSH
- Biological Transport MeSH
- Brefeldin A pharmacology MeSH
- Cell Membrane metabolism MeSH
- Endosomes metabolism MeSH
- Plant Epidermis cytology drug effects genetics metabolism MeSH
- Phenotype MeSH
- Plants, Genetically Modified MeSH
- Plant Roots cytology drug effects genetics metabolism MeSH
- Indoleacetic Acids metabolism MeSH
- Membrane Transport Proteins genetics metabolism MeSH
- Mutation MeSH
- Arabidopsis Proteins genetics metabolism MeSH
- Recombinant Fusion Proteins MeSH
- Body Patterning MeSH
- Seedlings cytology drug effects genetics metabolism MeSH
- trans-Golgi Network metabolism MeSH
- Vesicular Transport Proteins genetics metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- Names of Substances
- Brefeldin A MeSH
- EXO70A1 protein, Arabidopsis MeSH Browser
- Indoleacetic Acids MeSH
- Membrane Transport Proteins MeSH
- PIN1 protein, Arabidopsis MeSH Browser
- PIN2 protein, Arabidopsis MeSH Browser
- Arabidopsis Proteins MeSH
- Recombinant Fusion Proteins MeSH
- SEC8 protein, Arabidopsis MeSH Browser
- Vesicular Transport Proteins MeSH
In land plants polar auxin transport is one of the substantial processes guiding whole plant polarity and morphogenesis. Directional auxin fluxes are mediated by PIN auxin efflux carriers, polarly localized at the plasma membrane. The polarization of exocytosis in yeast and animals is assisted by the exocyst: an octameric vesicle-tethering complex and an effector of Rab and Rho GTPases. Here we show that rootward polar auxin transport is compromised in roots of Arabidopsis thaliana loss-of-function mutants in the EXO70A1 exocyst subunit. The recycling of PIN1 and PIN2 proteins from brefeldin-A compartments is delayed after the brefeldin-A washout in exo70A1 and sec8 exocyst mutants. Relocalization of PIN1 and PIN2 proteins after prolonged brefeldin-A treatment is largely impaired in these mutants. At the same time, however, plasma membrane localization of GFP:EXO70A1, and the other exocyst subunits studied (GFP:SEC8 and YFP:SEC10), is resistant to brefeldin-A treatment. In root cells of the exo70A1 mutant, a portion of PIN2 is internalized and retained in specific, abnormally enlarged, endomembrane compartments that are distinct from VHA-a1-labelled early endosomes or the trans-Golgi network, but are RAB-A5d positive. We conclude that the exocyst is involved in PIN1 and PIN2 recycling, and thus in polar auxin transport regulation.
References provided by Crossref.org
Plasma membrane phospholipid signature recruits the plant exocyst complex via the EXO70A1 subunit
Functional Specialization within the EXO70 Gene Family in Arabidopsis
Rab-dependent vesicular traffic affects female gametophyte development in Arabidopsis
The TOR-Auxin Connection Upstream of Root Hair Growth
EXO70A2 Is Critical for Exocyst Complex Function in Pollen Development
Developmental plasticity of Arabidopsis hypocotyl is dependent on exocyst complex function
Exocyst Subunit EXO70H4 Has a Specific Role in Callose Synthase Secretion and Silica Accumulation
RIN4 recruits the exocyst subunit EXO70B1 to the plasma membrane
Immunogold-EM analysis reveal brefeldin a-sensitive clusters of auxin in Arabidopsis root apex cells
EXO70C2 Is a Key Regulatory Factor for Optimal Tip Growth of Pollen
Inhibitors of plant hormone transport
Tethering Complexes in the Arabidopsis Endomembrane System
Endosidin2 targets conserved exocyst complex subunit EXO70 to inhibit exocytosis
