Candidate target genes for the Saccharomyces cerevisiae transcription factor, Yap2
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Alcohol Oxidoreductases biosynthesis genetics MeSH
- Chromatin Immunoprecipitation MeSH
- DNA, Fungal genetics MeSH
- Genes, Fungal * MeSH
- Oxidoreductases biosynthesis genetics MeSH
- Promoter Regions, Genetic MeSH
- Proteomics methods MeSH
- Gene Expression Regulation, Fungal * MeSH
- Saccharomyces cerevisiae Proteins biosynthesis genetics metabolism MeSH
- Saccharomyces cerevisiae genetics metabolism MeSH
- Transcription Factors metabolism MeSH
- Protein Binding MeSH
- Binding Sites MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alcohol Oxidoreductases MeSH
- CAD1 protein, S cerevisiae MeSH Browser
- DNA, Fungal MeSH
- Oxidoreductases MeSH
- Saccharomyces cerevisiae Proteins MeSH
- Transcription Factors MeSH
- YDL124W protein, S cerevisiae MeSH Browser
- YNL134C protein, S cerevisiae MeSH Browser
In Saccharomyces cerevisiae, the Yap family of basic leucine zipper (bZip) proteins contains eight members. The Yap family proteins are implicated in a variety of stress responses; among these proteins, Yap1 acts as a major regulator of oxidative stress responses. However, the functional roles of the remaining Yap family members are poorly understood. To elucidate the function of Yap2, we mined candidate target genes of Yap2 by proteomic analysis. Among the identified genes, FRM2 was previously identified as a target gene of Yap2, which confirmed the validity of our screening method. YNL134C and YDL124W were also identified as candidate Yap2 target genes. These genes were upregulated in strains overexpressing Yap2 and possess Yap2 target sequences in their promoter regions. Furthermore, chromatin immunoprecipitation assays showed that YNL134C and YDL124W have Yap2 binding motif. These data will help to elucidate the functional role of Yap2.
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