Detection and identification of globally distributed mycobacterial fish pathogens in some ornamental fish in India
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články
- MeSH
- DNA bakterií chemie genetika MeSH
- histocytochemie MeSH
- mikroskopie MeSH
- Mycobacterium chelonae klasifikace genetika izolace a purifikace fyziologie MeSH
- Mycobacterium fortuitum klasifikace genetika izolace a purifikace fyziologie MeSH
- mykobakteriózy mikrobiologie patologie veterinární MeSH
- nemoci ryb mikrobiologie patologie MeSH
- netuberkulózní mykobakterie klasifikace genetika izolace a purifikace fyziologie MeSH
- ribozomální DNA chemie genetika MeSH
- RNA ribozomální 16S genetika MeSH
- ryby mikrobiologie MeSH
- sekvenční analýza DNA MeSH
- techniky typizace bakterií MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Indie MeSH
- Názvy látek
- DNA bakterií MeSH
- ribozomální DNA MeSH
- RNA ribozomální 16S MeSH
Mycobacteriosis is a progressive disease of a wide range of wild and captive, marine and freshwater fish species. Conventional detection of fish Mycobacteria is based on histopathology, culture, and biochemical characteristics. The present study analyzed the occurrence of Mycobacteria in clinically ill ornamental fish of different species, from different places of India. In first group, 60 fish were examined for presence of granulomatous inflammation and acid-fast bacteria. Thirty-eight (63.34 %) fish were positive for granulomatous inflammations. Presences of acid-fast bacteria were detected in 27 (45 %) fish having granulomatous inflammation and in two (3.33 %) fish without granulomatous inflammation. In total, AFB were found in 29 (48.34 %) of the 60 fish examined. In second group, 20 fish having granulomatous inflammation, 12 (60 %) samples were positive using Ziehl-Neelsen (Z-N) staining and 11 (55 %) of them were culture positive. Eight (40 %) samples were Z-N negative but two (10 %) of them were culture positive. In total, 13 (65 %) of the 20 examined fish were culture positive. On the basis of biochemical tests and 16S rRNA sequencing, 13 isolates were identified: five as Mycobacterium fortuitum, five as Mycobacterium gordonae, and three as Mycobacterium chelonae. In comparison of two decontamination methods, 2 % HCl treatment was better than 4 % NaOH treatment. Mycobacteria recovery from decontaminated samples was significantly high on Lowenstein-Jensen medium compared to Middlebrook 7H11 agar and Stonebrink (SB) media. The disease is transmissible from fish to fish and also from fish to human, so the significance of Mycobacteria in ornamental fish should not be overlooked.
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Comp Med. 2000 Dec;50(6):666-72 PubMed
J Clin Microbiol. 1998 Jan;36(1):139-47 PubMed
Comp Med. 2001 Apr;51(2):171-5 PubMed
J Clin Microbiol. 2011 Jun;49(6):2296-303 PubMed
J Clin Microbiol. 2001 Feb;39(2):710-5 PubMed
Emerg Infect Dis. 2010 Feb;16(2):294-6 PubMed
BMC Public Health. 2011 May 16;11:320 PubMed
J Clin Microbiol. 1996 Dec;34(12):2963-7 PubMed
Dis Aquat Organ. 2004 Oct 21;61(1-2):41-51 PubMed
Clin Infect Dis. 2006 Jun 15;42(12):1756-63 PubMed
Can J Microbiol. 1966 Aug;12(4):715-23 PubMed
Emerg Infect Dis. 2001 Nov-Dec;7(6):1039-42 PubMed
Int J Syst Bacteriol. 1993 Jul;43(3):482-9 PubMed
Diagn Microbiol Infect Dis. 2000 Apr;36(4):225-35 PubMed
Can J Microbiol. 1979 Feb;25(2):217-26 PubMed
J Comp Pathol. 1990 Apr;102(3):335-44 PubMed
Vet Med (Praha). 1997 Apr;42(4):111-23 PubMed
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2095-2105 PubMed
Int J Tuberc Lung Dis. 2010 Sep;14(9):1176-80 PubMed
Vet Microbiol. 1997 Nov;58(2-4):229-37 PubMed
Vet Res Commun. 2004 Aug;28 Suppl 1:315-7 PubMed
Emerg Infect Dis. 2010 Oct;16(10):1576-83 PubMed
J Fish Dis. 2003 Apr;26(4):241-5 PubMed
J Clin Microbiol. 2002 Sep;40(9):3374-80 PubMed
Appl Environ Microbiol. 2002 Nov;68(11):5281-7 PubMed
Iran J Public Health. 2012;41(4):108-14 PubMed
