Getting into a weakened organism, non-tuberculosis mycobacteria (NTMB) contact not only with the cells of the microorganism but also with the microflora of the human body; however, these interactions are poorly understood. The purpose of this work was to study the effect of NTMB supernatants on the properties of conditionally pathogenic microorganisms in their interaction with red blood cells. We used strains of non-tuberculous mycobacteria Mycobacterium iranicum and M. rutilum, as well as strains of Staphylococcus epidermidis and Escherichia coli. Using the fluorescence in situ hybridisation (FISH) method, the processes of adhesion to the surface of erythrocytes and the intra-erythrocyte penetration of cells of S. epidermidis and E. coli under the influence of NTMB supernatants were studied. To study changes in the haemoglobin molecule under the action of the supernatants of NTMB, spectral analysis was performed. Statistical processing was performed using STATISTIKA 6.0. The results showed that the supernatants of M. iranicum and M. rutilum increased the adhesion of conditionally pathogenic bacteria with a low level of AntiHbA to the surface of erythrocytes by 3-4 times. It also increased the intra-erythrocyte penetration of cells of S. epidermidis and E. coli relative to the control values. As a result of studying the haemoglobin spectrum of erythrocytes under the influence of M. iranicum, a decrease in the optical density values of oxyhaemoglobin by a factor of 2 relative to the values in the control sample was noted. Thus, the supernatants of NTMB have a multidirectional effect on the interaction of opportunistic microorganisms with erythrocytes, increasing the adhesive activity and the penetration of cells into the erythrocytes, as well as reducing the optical density of oxyhaemoglobin.

• MeSH
• bakteriální adheze MeSH
• erytrocyty metabolismus   mikrobiologie   MeSH
• hemoglobiny metabolismus   MeSH
• lidé MeSH
• netuberkulózní mykobakterie fyziologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

We report a case of cavitary pulmonary disease caused by Mycobacterium shimoidei in 67-year-old female with history of asthma. Even though susceptibility testing was not available, choice of treatment regimen (streptomycin, rifampicin, ethambutol, and clarithromycin), based on a few cases with favorable outcome reported in the literature, resulted with an excellent clinical, microbiological, and radiological response. This is the first report of pulmonary disease caused by M. shimoidei, but also the first ever isolation of M. shimoidei in Croatia.

• MeSH
• antibakteriální látky aplikace a dávkování   MeSH
• atypické mykobakteriální infekce farmakoterapie   mikrobiologie   MeSH
• klarithromycin aplikace a dávkování   MeSH
• lidé MeSH
• netuberkulózní mykobakterie účinky léků   izolace a purifikace   fyziologie   MeSH
• plicní nemoci farmakoterapie   mikrobiologie   MeSH
• rifampin aplikace a dávkování   MeSH
• senioři MeSH
• streptomycin aplikace a dávkování   MeSH
• výsledek terapie MeSH
• Check Tag
• lidé MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• Geografické názvy
• Chorvatsko MeSH

Mycobacteriosis is a progressive disease of a wide range of wild and captive, marine and freshwater fish species. Conventional detection of fish Mycobacteria is based on histopathology, culture, and biochemical characteristics. The present study analyzed the occurrence of Mycobacteria in clinically ill ornamental fish of different species, from different places of India. In first group, 60 fish were examined for presence of granulomatous inflammation and acid-fast bacteria. Thirty-eight (63.34 %) fish were positive for granulomatous inflammations. Presences of acid-fast bacteria were detected in 27 (45 %) fish having granulomatous inflammation and in two (3.33 %) fish without granulomatous inflammation. In total, AFB were found in 29 (48.34 %) of the 60 fish examined. In second group, 20 fish having granulomatous inflammation, 12 (60 %) samples were positive using Ziehl-Neelsen (Z-N) staining and 11 (55 %) of them were culture positive. Eight (40 %) samples were Z-N negative but two (10 %) of them were culture positive. In total, 13 (65 %) of the 20 examined fish were culture positive. On the basis of biochemical tests and 16S rRNA sequencing, 13 isolates were identified: five as Mycobacterium fortuitum, five as Mycobacterium gordonae, and three as Mycobacterium chelonae. In comparison of two decontamination methods, 2 % HCl treatment was better than 4 % NaOH treatment. Mycobacteria recovery from decontaminated samples was significantly high on Lowenstein-Jensen medium compared to Middlebrook 7H11 agar and Stonebrink (SB) media. The disease is transmissible from fish to fish and also from fish to human, so the significance of Mycobacteria in ornamental fish should not be overlooked.

• MeSH
• DNA bakterií chemie   genetika   MeSH
• histocytochemie MeSH
• mikroskopie MeSH
• Mycobacterium chelonae klasifikace   genetika   izolace a purifikace   fyziologie   MeSH
• Mycobacterium fortuitum klasifikace   genetika   izolace a purifikace   fyziologie   MeSH
• mykobakteriózy mikrobiologie   patologie   veterinární   MeSH
• nemoci ryb mikrobiologie   patologie   MeSH
• netuberkulózní mykobakterie klasifikace   genetika   izolace a purifikace   fyziologie   MeSH
• ribozomální DNA chemie   genetika   MeSH
• RNA ribozomální 16S genetika   MeSH
• ryby mikrobiologie   MeSH
• sekvenční analýza DNA MeSH
• techniky typizace bakterií MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Indie MeSH