Adenosine modulates LPS-induced cytokine production in porcine monocytes
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23388525
DOI
10.1016/j.cyto.2012.12.026
PII: S1043-4666(13)00005-7
Knihovny.cz E-resources
- MeSH
- Adenosine-5'-(N-ethylcarboxamide) pharmacology MeSH
- Adenosine pharmacology MeSH
- Purinergic P1 Receptor Agonists pharmacology MeSH
- Purinergic P1 Receptor Antagonists pharmacology MeSH
- CD163 Antigen MeSH
- Antigens, Differentiation, Myelomonocytic metabolism MeSH
- Antigens, CD metabolism MeSH
- Cytokines biosynthesis genetics MeSH
- Lipopolysaccharides pharmacology MeSH
- RNA, Messenger genetics metabolism MeSH
- Monocytes drug effects metabolism MeSH
- Receptors, Purinergic P1 genetics metabolism MeSH
- Receptors, Cell Surface metabolism MeSH
- Gene Expression Regulation drug effects MeSH
- Sus scrofa metabolism MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenosine-5'-(N-ethylcarboxamide) MeSH
- Adenosine MeSH
- Purinergic P1 Receptor Agonists MeSH
- Purinergic P1 Receptor Antagonists MeSH
- CD163 Antigen MeSH
- Antigens, Differentiation, Myelomonocytic MeSH
- Antigens, CD MeSH
- Cytokines MeSH
- Lipopolysaccharides MeSH
- RNA, Messenger MeSH
- Receptors, Purinergic P1 MeSH
- Receptors, Cell Surface MeSH
Adenosine plays an important role during inflammation, particularly through modulation of monocyte function. The objective of the present study was to evaluate the effect of synthetic adenosine analogs on cytokine production by porcine monocytes. The LPS-stimulated cytokine production was measured by flow cytometry and quantitative real-time PCR. Adenosine receptor expression was measured by quantitative real-time PCR. The present study demonstrates that adenosine analog N-ethylcarboxyamidoadenosine (NECA) down-regulates TNF-α production and up-regulates IL-8 production by LPS-stimulated porcine monocytes. The effect was more pronounced in CD163(-) subset of monocytes compared to the CD163(+) subset. Although both monocyte subsets express mRNA for A1, A2A, A2B and A3 adenosine receptors, the treatment of monocytes with various adenosine receptor agonists and antagonists proved that the effect of adenosine is mediated preferentially via A2A adenosine receptor. Moreover, the study suggests that the effect of NECA on porcine monocytes alters the levels of the cytokines which could play a role in the differentiation of naive T cells into Th17 cells. The results suggest that adenosine plays an important role in modulation of cytokine production by porcine monocytes.
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