A polymerase chain reaction-based approach for evaluation of telomere-associated sequences and interstitial telomeric sequences
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23583821
DOI
10.1016/j.ab.2013.03.034
PII: S0003-2697(13)00162-0
Knihovny.cz E-resources
- MeSH
- Arabidopsis genetics MeSH
- DNA, Plant genetics MeSH
- Polymerase Chain Reaction methods MeSH
- Base Sequence MeSH
- Telomere genetics MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Plant MeSH
Telomere minisatellites could be present in both terminal and internal chromosomal regions. We monitored the progress of BAL-31 nuclease digestion on Arabidopsis thaliana genomic DNA prepared by standard isolation techniques to verify its cleavage at terminal and internal genomic regions. A subtelomeric position of candidate sequences was validated using conventional polymerase chain reaction (PCR), combining the C-strand-specific telomeric primer with a subtelomeric reverse primer, and confirmed by quantitative PCR (qPCR) using sequence-specific primer pairs on DNA samples after BAL-31 digestion. qPCR amplification showed a gradual decrease in subtelomeric sequence signals, in contrast to interstitial telomeric sequences from pericentromere and control sequences.
References provided by Crossref.org
