Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important wheat diseases in the world. In this study, a single dominant powdery mildew resistance gene MlIW172 was identified in the IW172 wild emmer accession and mapped to the distal region of chromosome arm 7AL (bin7AL-16-0.86-0.90) via molecular marker analysis. MlIW172 was closely linked with the RFLP probe Xpsr680-derived STS marker Xmag2185 and the EST markers BE405531 and BE637476. This suggested that MlIW172 might be allelic to the Pm1 locus or a new locus closely linked to Pm1. By screening genomic BAC library of durum wheat cv. Langdon and 7AL-specific BAC library of hexaploid wheat cv. Chinese Spring, and after analyzing genome scaffolds of Triticum urartu containing the marker sequences, additional markers were developed to construct a fine genetic linkage map on the MlIW172 locus region and to delineate the resistance gene within a 0.48 cM interval. Comparative genetics analyses using ESTs and RFLP probe sequences flanking the MlIW172 region against other grass species revealed a general co-linearity in this region with the orthologous genomic regions of rice chromosome 6, Brachypodium chromosome 1, and sorghum chromosome 10. However, orthologous resistance gene-like RGA sequences were only present in wheat and Brachypodium. The BAC contigs and sequence scaffolds that we have developed provide a framework for the physical mapping and map-based cloning of MlIW172.

Department of Plant Sciences University of California Davis Davis California United States of America

Institute of Experimental Botany Centre of Plant Structural and Functional Genomics Olomouc Czech Republic

Murdoch University Perth Western Australia Australia

State Key Laboratory for Agrobiotechnology Beijing Key Laboratory of Crop Genetic Improvement Key Laboratory of Crop Heterosis Research and Utilization Ministry of Education China Agricultural University Beijing China

State Key Laboratory for Agrobiotechnology Beijing Key Laboratory of Crop Genetic Improvement Key Laboratory of Crop Heterosis Research and Utilization Ministry of Education China Agricultural University Beijing China; Agriculture University of Beijing Beijing China

State Key Laboratory for Agrobiotechnology Beijing Key Laboratory of Crop Genetic Improvement Key Laboratory of Crop Heterosis Research and Utilization Ministry of Education China Agricultural University Beijing China; Maize Research Center Beijing Academy of Agricultural and Forestry Sciences Beijing China

State Key Laboratory of Plant Cell and Chromosome Engineering Institutes of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China

USDA ARS West Regional Research Center Albany California United States of America

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Identification, High-Density Mapping, and Characterization of New Major Powdery Mildew Resistance Loci From the Emmer Wheat Landrace GZ1

A highly differentiated region of wheat chromosome 7AL encodes a Pm1a immune receptor that recognizes its corresponding AvrPm1a effector from Blumeria graminis

Molecular organization and comparative analysis of chromosome 5B of the wild wheat ancestor Triticum dicoccoides