Treating spinal cord injury in rats with a combination of human fetal neural stem cells and hydrogels modified with serotonin
Jazyk angličtina Země Polsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23595287
DOI
10.55782/ane-2013-1925
PII: 1925
Knihovny.cz E-zdroje
- MeSH
- atrofie etiologie terapie MeSH
- buněčná diferenciace MeSH
- cholinesterasy metabolismus MeSH
- fetální kmenové buňky fyziologie MeSH
- jizva farmakoterapie etiologie MeSH
- krysa rodu Rattus MeSH
- lidé MeSH
- lokomoce účinky léků fyziologie MeSH
- modely nemocí na zvířatech MeSH
- myelinové proteiny metabolismus MeSH
- nervové kmenové buňky fyziologie MeSH
- PEG-DMA hydrogel aplikace a dávkování MeSH
- poranění míchy farmakoterapie chirurgie MeSH
- potkani Wistar MeSH
- proliferace buněk MeSH
- proteiny nervové tkáně metabolismus MeSH
- serotonin terapeutické užití MeSH
- transfekce MeSH
- transplantace kmenových buněk * MeSH
- zelené fluorescenční proteiny genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cholinesterasy MeSH
- Mobp protein, rat MeSH Prohlížeč
- myelinové proteiny MeSH
- PEG-DMA hydrogel MeSH
- proteiny nervové tkáně MeSH
- serotonin MeSH
- zelené fluorescenční proteiny MeSH
Currently, there is no effective strategy for the treatment of spinal cord injury (SCI). A combination of biomaterials and stem cell therapy seems to be a promising approach to increase regenerative potential after SCI. We evaluated the use of a cellpolymer construct based on a combination of the conditionally immortalized spinal progenitor cell line SPC-01_GFP3, derived from human fetal spinal cord tissue, with a serotonin-modified poly(2-hydroxyethyl methacrylate) hydrogel (pHEMA-5HT). We compared the effect of treatment with a pHEMA-5HT hydrogel seeded with SPC-01_GFP3 cells, treatment with a pHEMA-5HT only and no treatment on functional outcome and tissue reconstruction in hemisected rats. Prior to transplantation the cell-polymer construct displayed a high potential to support the growth, proliferation and differentiation of SPC-01 cells in vitro. One month after surgery, combined hydrogel-cell treatment reduced astrogliosis and tissue atrophy and increased axonal and blood vessel ingrowth into the implant; however, two months later only the ingrowth of blood vessels remained increased. SPC-01_GFP3 cells survived well in vivo and expressed advanced markers of neuronal differentiation. However, a majority of the transplanted cells migrated out of the lesion and only rarely remained in the hydrogel. No differences among the groups in motor or sensory recovery were observed. Despite the support of the hydrogel as a cell carrier in vitro, and good results in vivo one month postsurgery, there was only a small effect on long term recovery, mainly due to the limited ability of the hydrogels to support the in vivo growth and differentiation of cells within the implant. Further modifications will be necessary to achieve stable long term improvement in functional outcome.
Citace poskytuje Crossref.org
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