Potentiation of inhibitory synaptic transmission by extracellular ATP in rat suprachiasmatic nuclei
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
23637193
PubMed Central
PMC6618955
DOI
10.1523/jneurosci.4682-12.2013
PII: 33/18/8035
Knihovny.cz E-zdroje
- MeSH
- adenosintrifosfát farmakologie MeSH
- antagonisté excitačních aminokyselin farmakologie MeSH
- biofyzikální jevy účinky léků MeSH
- blokátory sodíkových kanálů farmakologie MeSH
- GABA farmakologie MeSH
- inhibitory agregace trombocytů farmakologie MeSH
- krysa rodu Rattus MeSH
- kultivované buňky MeSH
- messenger RNA metabolismus MeSH
- metoda terčíkového zámku MeSH
- nervový přenos účinky léků MeSH
- nervový útlum účinky léků MeSH
- neurony účinky léků MeSH
- novorozená zvířata MeSH
- nucleus suprachiasmaticus cytologie MeSH
- potkani Wistar MeSH
- purinergní látky farmakologie MeSH
- purinergní receptory P2X genetika metabolismus MeSH
- regulace genové exprese účinky léků MeSH
- synaptické potenciály účinky léků MeSH
- techniky in vitro MeSH
- tetrodotoxin farmakologie MeSH
- vápník metabolismus MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adenosintrifosfát MeSH
- antagonisté excitačních aminokyselin MeSH
- blokátory sodíkových kanálů MeSH
- GABA MeSH
- inhibitory agregace trombocytů MeSH
- messenger RNA MeSH
- purinergní látky MeSH
- purinergní receptory P2X MeSH
- tetrodotoxin MeSH
- vápník MeSH
The hypothalamic suprachiasmatic nuclei (SCN), the circadian master clock in mammals, releases ATP in a rhythm, but the role of extracellular ATP in the SCN is still unknown. In this study, we examined the expression and function of ATP-gated P2X receptors (P2XRs) in the SCN neurons of slices isolated from the brain of 16- to 20-day-old rats. Quantitative RT-PCR showed that the SCN contains mRNA for P2X 1-7 receptors and several G-protein-coupled P2Y receptors. Among the P2XR subunits, the P2X2 > P2X7 > P2X4 mRNAs were the most abundant. Whole-cell patch-clamp recordings from SCN neurons revealed that extracellular ATP application increased the frequency of spontaneous GABAergic IPSCs without changes in their amplitudes. The effect of ATP appears to be mediated by presynaptic P2X2Rs because ATPγS and 2MeS-ATP mimics, while the P2XR antagonist PPADS blocks, the observed enhancement of the frequency of GABA currents. There were significant differences between two SCN regions in that the effect of ATP was higher in the ventrolateral subdivision, which is densely innervated from outside the SCN. Little evidence was found for the presence of P2XR channels in somata of SCN neurons as P2X2R immunoreactivity colocalized with synapsin and ATP-induced current was observed in only 7% of cells. In fura-2 AM-loaded slices, BzATP as well as ADP stimulated intracellular Ca(2+) increase, indicating that the SCN cells express functional P2X7 and P2Y receptors. Our data suggest that ATP activates presynaptic P2X2Rs to regulate inhibitory synaptic transmission within the SCN and that this effect varies between regions.
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