Cell wall components of Leptosphaeria maculans enhance resistance of Brassica napus
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23638999
DOI
10.1021/jf401221v
Knihovny.cz E-resources
- MeSH
- Ascomycota chemistry growth & development immunology MeSH
- Aspergillus niger enzymology MeSH
- Brassica napus drug effects immunology metabolism microbiology MeSH
- Cell Wall chemistry MeSH
- Cell Extracts chemistry isolation & purification pharmacology MeSH
- Down-Regulation MeSH
- Fungal Proteins analysis chemistry metabolism MeSH
- Glycoside Hydrolases biosynthesis genetics metabolism MeSH
- Glycosides analysis chemistry metabolism MeSH
- Hydrolysis MeSH
- Mycelium chemistry growth & development immunology MeSH
- Disease Resistance drug effects MeSH
- Oligosaccharides analysis chemistry metabolism MeSH
- Peptide Fragments analysis chemistry metabolism MeSH
- Hydrogen Peroxide metabolism MeSH
- Fungicides, Industrial chemistry isolation & purification pharmacology MeSH
- Plant Proteins biosynthesis genetics metabolism MeSH
- Seedlings drug effects immunology metabolism microbiology MeSH
- Up-Regulation drug effects MeSH
- Chemistry, Agricultural methods MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cell Extracts MeSH
- Fungal Proteins MeSH
- Glycoside Hydrolases MeSH
- Glycosides MeSH
- Oligosaccharides MeSH
- pathogenesis-related proteins, plant MeSH Browser
- Peptide Fragments MeSH
- Hydrogen Peroxide MeSH
- Fungicides, Industrial MeSH
- Plant Proteins MeSH
Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans , a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide accumulation, and enhanced resistance of B. napus plants toward infection by L. maculans. Elicitation activity significantly decreased after treatment of a crude extract with α- or β-glucanase. Monosaccharide composition analysis of a crude extract purified by ion-exchange chromatography revealed glucose (∼58 mol %), mannose (∼22 mol %), and galactose (∼18 mol %) as the major sugars. FT-IR and NMR spectra confirmed the presence of both carbohydrate and polypeptide components in the purified product. Correlation NMR experiments defined trisaccharide bound to O-3 of serine residue α-D-Glcp-(1→2)-β-D-Galf-(1→6)-α-D-Manp-(1→3)-L-Ser. Terminal α-D-Glcp and (1→6)-β-D-glucan were also detected. The obtained results strongly support the conclusion that these carbohydrates induce defense response in B. napus plants.
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