Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans , a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide accumulation, and enhanced resistance of B. napus plants toward infection by L. maculans. Elicitation activity significantly decreased after treatment of a crude extract with α- or β-glucanase. Monosaccharide composition analysis of a crude extract purified by ion-exchange chromatography revealed glucose (∼58 mol %), mannose (∼22 mol %), and galactose (∼18 mol %) as the major sugars. FT-IR and NMR spectra confirmed the presence of both carbohydrate and polypeptide components in the purified product. Correlation NMR experiments defined trisaccharide bound to O-3 of serine residue α-D-Glcp-(1→2)-β-D-Galf-(1→6)-α-D-Manp-(1→3)-L-Ser. Terminal α-D-Glcp and (1→6)-β-D-glucan were also detected. The obtained results strongly support the conclusion that these carbohydrates induce defense response in B. napus plants.
- MeSH
- Ascomycota chemie růst a vývoj imunologie MeSH
- Aspergillus niger enzymologie MeSH
- Brassica napus účinky léků imunologie metabolismus mikrobiologie MeSH
- buněčná stěna chemie MeSH
- buněčné extrakty chemie izolace a purifikace farmakologie MeSH
- chemie zemědělská metody MeSH
- down regulace MeSH
- fungální proteiny analýza chemie metabolismus MeSH
- fungicidy průmyslové chemie izolace a purifikace farmakologie MeSH
- glykosidhydrolasy biosyntéza genetika metabolismus MeSH
- glykosidy analýza chemie metabolismus MeSH
- hydrolýza MeSH
- mycelium chemie růst a vývoj imunologie MeSH
- odolnost vůči nemocem účinky léků MeSH
- oligosacharidy analýza chemie metabolismus MeSH
- peptidové fragmenty analýza chemie metabolismus MeSH
- peroxid vodíku metabolismus MeSH
- rostlinné proteiny biosyntéza genetika metabolismus MeSH
- semenáček účinky léků imunologie metabolismus mikrobiologie MeSH
- upregulace účinky léků MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
