Helicobacter pylori (HP) is considered a major gastric pathogen with oncogenic potential. The aim of this study was to determine whether HP is present in oropharyngeal lymphoid tissue and whether oropharyngeal HP strains carry virulence factor genes known to be involved in gastric carcinogenesis. The study included 104 subjects (41 patients with tonsillar carcinoma, 38 with chronic tonsillitis and 25 with obstructive sleep apnoea syndrome--OSAS). Detection of specific serum anti-HP antibodies was performed with an ELISA. The presence of HP in tissue was determined by culture and real-time PCR. Detection of virulence factors genes was also performed. Specific antibodies were found in 78.05% of tumour cases, 34.21% of chronic tonsillitis cases, and 72.0% of OSAS cases. The presence of HP in the tissue was detected in 73.91% of tonsillar tumours, 70.0% of tonsillitis cases, and 69.23% of OSAS specimens. The results of the virulence factor gene analysis showed the majority of the s1b (52.4%) and m2 (59.5%) alleles of vacA gene and limited abundance of cagA gene (12.5%). Results confirm that HP may colonise oropharyngeal lymphoid tissue. Oropharyngeal HP colonisation was frequently found in the oropharyngeal cancer group and in patients with benign oropharyngeal diseases. A virulence factor gene analysis showed differences from the predominant strains most commonly found in the stomach. The strains obtained from the oropharynx differed primarily by the lower abundance of the cagA gene and carried the less virulent vacA gene allele combination.

Department of Otorhinolaryngology Head and Neck Surgery 1st Faculty of Medicine Charles University Prague and University Hospital Motol 5 Úvalu 84 150 06 Prague 5 Czech Republic

Zobrazit více v PubMed

Am J Med. 1996 May 20;100(5A):12S-17S; discussion 17S-18S PubMed

Cas Lek Cesk. 2008;147(5):255-7 PubMed

Helicobacter. 2006 Feb;11(1):56-65 PubMed

Dig Dis Sci. 2002 May;47(5):978-86 PubMed

Microbiol Immunol. 2000;44(5):385-8 PubMed

Eur J Oral Sci. 2008 Aug;116(4):297-304 PubMed

J Med Microbiol. 2002 Apr;51(4):312-317 PubMed

Epidemiol Rev. 2000;22(2):283-97 PubMed

Lancet. 2003 Oct 11;362(9391):1231-3 PubMed

Arch Otolaryngol Head Neck Surg. 2003 Nov;129(11):1225-9 PubMed

FEMS Immunol Med Microbiol. 2009 Jul;56(2):112-5 PubMed

J Clin Microbiol. 1998 May;36(5):1271-6 PubMed

N Engl J Med. 2001 Sep 13;345(11):784-9 PubMed

Aliment Pharmacol Ther. 2001 Sep;15(9):1271-90 PubMed

Clin Otolaryngol Allied Sci. 2001 Dec;26(6):505-9 PubMed

J Clin Microbiol. 2004 Oct;42(10):4512-8 PubMed

Curr Opin Gastroenterol. 2004 Jan;20(1):10-5 PubMed

Helicobacter. 2008 Oct;13 Suppl 1:28-34 PubMed

Gastroenterology. 2003 Dec;125(6):1636-44 PubMed

Clin Sci (Lond). 2006 Mar;110(3):305-14 PubMed

Prague Med Rep. 2012;113(3):231-9 PubMed

Gastroenterology. 1999 Apr;116(4):823-30 PubMed

Int J Pediatr Otorhinolaryngol. 2011 Apr;75(4):568-72 PubMed

Am J Med. 1996 May 20;100(5A):2S-9S; discussion 9S-11S PubMed

J Biol Chem. 1995 Jul 28;270(30):17771-7 PubMed

Hum Pathol. 2007 Dec;38(12):1788-97 PubMed

FEMS Microbiol Lett. 1997 Jul 15;152(2):355-61 PubMed

J Med Microbiol. 1999 Mar;48(3):317-322 PubMed

Folia Biol (Praha). 2008;54(2):33-9 PubMed

Clin Diagn Lab Immunol. 2003 Jan;10(1):116-24 PubMed

Crit Rev Oral Biol Med. 2003;14(3):226-33 PubMed

Int J Cancer. 2000 Aug 1;87(3):322-7 PubMed

Rev Med Chil. 2001 Oct;129(10):1147-53 PubMed

Folia Microbiol (Praha). 2007;52(1):91-4 PubMed

Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):15136-41 PubMed

J Clin Microbiol. 1999 Apr;37(4):1203-5 PubMed

Salivary glands - a new site of Helicobacter pylori occurrence

Genome of Helicobacter pylori and Serotype of HPV Detected in Oropharyngeal and Laryngeal Cancer and Chronic Inflammation Patients