Antiproliferative effect of benzimidazole anthelmintics albendazole, ricobendazole, and flubendazole in intestinal cancer cell lines
Jazyk angličtina Země Velká Británie, Anglie Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
- MeSH
- adenokarcinom farmakoterapie MeSH
- albendazol analogy a deriváty farmakologie MeSH
- anthelmintika farmakologie MeSH
- antitumorózní látky fytogenní farmakologie MeSH
- antitumorózní látky farmakologie MeSH
- chemorezistence účinky léků MeSH
- G2 fáze účinky léků MeSH
- inhibiční koncentrace 50 MeSH
- lidé MeSH
- mebendazol analogy a deriváty farmakologie MeSH
- mikrotubuly účinky léků MeSH
- modulátory tubulinu farmakologie MeSH
- nádorové buněčné linie MeSH
- paclitaxel farmakologie MeSH
- proliferace buněk účinky léků MeSH
- stabilita proteinů účinky léků MeSH
- střevní nádory farmakoterapie MeSH
- synergismus léků MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- albendazol MeSH
- albendazole sulfoxide MeSH Prohlížeč
- anthelmintika MeSH
- antitumorózní látky fytogenní MeSH
- antitumorózní látky MeSH
- flubendazole MeSH Prohlížeč
- mebendazol MeSH
- modulátory tubulinu MeSH
- paclitaxel MeSH
This study aimed to test the antiproliferative effect of three benzimidazole anthelmintics in intestinal cancer cells and to investigate whether these drugs, which inhibit tubulin polymerization, can potentiate the efficacy of the microtubule-stabilizing drug paclitaxel (PTX). Four intestinal cancer cell lines, SW480, SW620, HCT8, and Caco2, with different origins and growth characteristics were used. The antiproliferative effect of albendazole (ABZ), ricobendazole (RBZ), flubendazole (FLU), and their combinations with PTX was tested using three different end-point viability assays, cell cycle distribution analysis, and the x-CELLigence System for real-time cell analysis. ABZ and FLU inhibited cell proliferation significantly in a concentration-dependent and time-dependent manner through cell arrest in the G2/M phase. RBZ was not effective at any concentration tested. The cell lines differed in sensitivity to FLU and ABZ, with HCT8 being the most sensitive, showing IC₅₀ values for ABZ and FLU that reached 0.3 and 0.9 μmol/l, respectively. Combinations of PTX+ABZ and PTX+FLU decreased cell viability more effectively when compared with treatment with individual drugs alone. The anthelmintic benzimidazole drugs ABZ and FLU show a significant cytostatic effect and potentiate the efficacy of PTX in intestinal cancer cells.
Citace poskytuje Crossref.org
Biological Activity and Molecular Structures of Bis(benzimidazole) and Trithiocyanurate Complexes
