Defining the selectivity of processes along the auxin response chain: a study using auxin analogues
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
23914741
DOI
10.1111/nph.12437
Knihovny.cz E-resources
- Keywords
- 2,4-dichlorophenoxyacetic acid (2,4-D), auxin analogues, auxin signalling, auxin transport, indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), naphthalene-1-acetic acid (NAA),
- MeSH
- Biological Transport, Active drug effects MeSH
- Arabidopsis drug effects genetics metabolism MeSH
- Cell Membrane drug effects metabolism MeSH
- Cell Division drug effects MeSH
- Endocytosis drug effects genetics MeSH
- Plant Roots drug effects growth & development MeSH
- Indoleacetic Acids pharmacology MeSH
- Mutation genetics MeSH
- Promoter Regions, Genetic genetics MeSH
- Arabidopsis Proteins genetics metabolism MeSH
- Gene Expression Regulation, Plant drug effects MeSH
- Plant Cells drug effects metabolism MeSH
- Seedlings drug effects growth & development MeSH
- Suspensions MeSH
- Nicotiana cytology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Indoleacetic Acids MeSH
- Arabidopsis Proteins MeSH
- Suspensions MeSH
The mode of action of auxin is based on its non-uniform distribution within tissues and organs. Despite the wide use of several auxin analogues in research and agriculture, little is known about the specificity of different auxin-related transport and signalling processes towards these compounds. Using seedlings of Arabidopsis thaliana and suspension-cultured cells of Nicotiana tabacum (BY-2), the physiological activity of several auxin analogues was investigated, together with their capacity to induce auxin-dependent gene expression, to inhibit endocytosis and to be transported across the plasma membrane. This study shows that the specificity criteria for different auxin-related processes vary widely. Notably, the special behaviour of some synthetic auxin analogues suggests that they might be useful tools in investigations of the molecular mechanism of auxin action. Thus, due to their differential stimulatory effects on DR5 expression, indole-3-propionic (IPA) and 2,4,5-trichlorophenoxy acetic (2,4,5-T) acids can serve in studies of TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALLING F-BOX (TIR1/AFB)-mediated auxin signalling, and 5-fluoroindole-3-acetic acid (5-F-IAA) can help to discriminate between transcriptional and non-transcriptional pathways of auxin signalling. The results demonstrate that the major determinants for the auxin-like physiological potential of a particular compound are very complex and involve its chemical and metabolic stability, its ability to distribute in tissues in a polar manner and its activity towards auxin signalling machinery.
References provided by Crossref.org
No Time for Transcription-Rapid Auxin Responses in Plants
Mechanistic framework for cell-intrinsic re-establishment of PIN2 polarity after cell division
Advances in Understanding the Mechanism of Action of the Auxin Permease AUX1
What Has Been Seen Cannot Be Unseen-Detecting Auxin In Vivo
