Auxin is a key plant regulatory molecule, which acts upon a plethora of cellular processes, including those related to cell differentiation and elongation. Despite the stunning progress in all disciplines of auxin research, the mechanisms of auxin-mediated rapid promotion of cell expansion and underlying rearrangement of cell wall components are poorly understood. This is partly due to the limitations of current methodologies for probing auxin. Here we describe a click chemistry-based approach, using an azido derivative of indole-3-propionic acid. This compound is as an active auxin analogue, which can be tagged in situ. Using this new tool, we demonstrate the existence of putative auxin binding sites in the cell walls of expanding/elongating cells. These binding sites are of protein nature but are distinct from those provided by the extensively studied AUXIN BINDING PROTEIN 1 (ABP1). Using immunohistochemistry, we have shown the apoplastic presence of endogenous auxin epitopes recognised by an anti-IAA antibody. Our results are intriguingly in line with previous observations suggesting some transcription-independent (non-genomic) activity of auxin in cell elongation.

CEITEC Masaryk University Kamenice 5 CZ 625 00 Brno Czechia

Department of Chemistry University of Copenhagen Thorvaldsensvej 40 1871 Frederiksberg C Denmark

Department of Plant and Environmental Sciences University of Copenhagen Thorvaldsensvej 40 1871 Frederiksberg C Denmark

Laboratory of Hormonal Regulations in Plants Institute of Experimental Botany Czech Academy of Sciences Rozvojová 263 CZ 165 02 Prague Czechia

School of Agriculture Food and Rural Development Newcastle University Newcastle upon Tyne NE1 7RU UK

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