Formation of superoxide anion and carbon-centered radicals by photosystem II under high light and heat stress-EPR spin-trapping study
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Electron Spin Resonance Spectroscopy MeSH
- Photochemical Processes MeSH
- Photosystem II Protein Complex chemistry metabolism MeSH
- Oxygen chemistry metabolism MeSH
- Oxidation-Reduction MeSH
- Spin Trapping methods MeSH
- Spinacia oleracea MeSH
- Superoxides chemistry metabolism MeSH
- Light MeSH
- Free Radicals chemistry metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Photosystem II Protein Complex MeSH
- Oxygen MeSH
- Superoxides MeSH
- Free Radicals MeSH
In this study, electron paramagnetic resonance spin-trapping spectroscopy was used to study the light-induced production of superoxide anion (O2 (•-)) and carbon-centered (R(•)) radicals by Photosystem II (PSII). It is evidenced here that exposure of PSII membranes to high light (2,000 μmol photons m(-2) s(-1)) or heat (47 °C) treatments prior to the illumination suppressed O2 (•-) production, while R(•) was formed. Formation of R(•) in the both high light- and heat-treated PSII membranes was enhanced by DCMU. Removal of molecular oxygen by glucose/glucose oxidase/catalase system and O2 (•-) scavenging by exogenous superoxide dismutase completely suppressed carbon-centered radical formation. It is proposed here that the oxidation of polyunsaturated fatty acids and amino acids by O2 (•-) on the electron acceptor side of PSII results in the formation of R(•), known to initiate a cascade reaction leading to the lipid peroxidation and protein degradation, respectively.
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J Biol Chem. 1991 Aug 15;266(23):15091-8 PubMed
Photosynth Res. 1994 Feb;39(2):191-9 PubMed
Biol Chem. 2002 May;383(5):813-20 PubMed
J Exp Biol. 1998 Apr;201(Pt 8):1203-9 PubMed
Biochem J. 1997 May 15;324 ( Pt 1):1-18 PubMed
Biochem J. 2006 Jul 15;397(2):321-7 PubMed
Biochemistry. 2004 Jun 1;43(21):6783-92 PubMed
Biochim Biophys Acta. 2009 Oct;1787(10):1151-60 PubMed
Biochim Biophys Acta. 2012 Jan;1817(1):218-31 PubMed
Biochemistry. 1998 Nov 17;37(46):16262-9 PubMed
Free Radic Biol Med. 2000 Feb 1;28(3):403-8 PubMed
Plant Physiol. 2006 Jun;141(2):391-6 PubMed
Annu Rev Plant Biol. 2004;55:373-99 PubMed
Photosynth Res. 1994 Aug;41(2):327-38 PubMed
FEBS Lett. 2000 May 4;473(1):58-62 PubMed
FEBS Lett. 1999 Sep 3;457(3):348-52 PubMed
