Benzofurazane as a new redox label for electrochemical detection of DNA: towards multipotential redox coding of DNA bases
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- DNA polymerase, electrochemistry, nucleoside triphosphates, sequencing, voltammetry,
- MeSH
- Benzofurans chemistry MeSH
- DNA-Directed DNA Polymerase metabolism MeSH
- DNA analysis MeSH
- Electrochemical Techniques * MeSH
- Nucleosides chemistry MeSH
- Oxidation-Reduction MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- benzofuran MeSH Browser
- Benzofurans MeSH
- DNA-Directed DNA Polymerase MeSH
- DNA MeSH
- Nucleosides MeSH
Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. In combination with nitrophenyl and aminophenyl labels, we have successfully developed a three-potential coding of DNA bases and have explored the relevant electrochemical potentials. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.
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