Juvenile hormone (JH), a sesquiterpenoid produced by the insect corpus allatum gland (CA), prevents metamorphosis in larvae and stimulates vitellogenesis in adult females. Whether the same JH signaling pathway regulates both processes is presently unknown. Here, we employ the robust JH response during reproduction and development of the linden bug, Pyrrhocoris apterus, to compare the function of key JH-signaling genes encoding the JH receptor, Methoprene-tolerant (Met), its binding partner Taiman (Tai), and a JH-inducible protein, Krüppel-homolog 1 (Kr-h1). RNA interference (RNAi) with Met or Tai, but not Kr-h1, blocked ovarian development and suppressed vitellogenin gene expression in the fat body of females raised under reproduction-inducing conditions. Loss of Met and Tai matched the effects of CA ablation or the natural absence of JH during reproductive diapause. Stimulation of vitellogenesis by treatment of diapausing females with a JH mimic methoprene also required both Met and Tai in the fat body, whereas Kr-h1 RNAi had no effect. Therefore, the Met-Tai complex likely functions as a JH receptor during vitellogenesis. In contrast to Met and Kr-h1 that are both required for JH to prevent precocious metamorphosis in P. apterus larvae, removal of Tai disrupted larval ecdysis without causing premature adult development. Our results show that while Met operates during metamorphosis in larvae and reproduction in adult females, its partner Tai is only required for the latter. The diverse functions of JH thus likely rely on a common receptor whose actions are modulated by distinct components.

Biology Center Academy of Sciences of the Czech Republic 37005 Ceske Budejovice Czech Republic

Biology Center Academy of Sciences of the Czech Republic 37005 Ceske Budejovice Czech Republic; Animal Food and Health Sciences Division Commonwealth Scientific and Industrial Research Organization North Ryde NSW 2113 Australia

Biology Center Academy of Sciences of the Czech Republic 37005 Ceske Budejovice Czech Republic; Department of Molecular Biology Faculty of Sciences University of South Bohemia 37005 Ceske Budejovice Czech Republic

References provided by Crossref.org

Impact of photoperiod and functional clock on male diapause in cryptochrome and pdf mutants in the linden bug Pyrrhocoris apterus

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Loss of Timeless Underlies an Evolutionary Transition within the Circadian Clock

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Light and Temperature Synchronizes Locomotor Activity in the Linden Bug, Pyrrhocoris apterus

Genetic Evidence for Function of the bHLH-PAS Protein Gce/Met As a Juvenile Hormone Receptor