Fibroblast growth factor-21 is expressed in neonatal and pheochromocytoma-induced adult human brown adipose tissue
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
24369918
DOI
10.1016/j.metabol.2013.11.014
PII: S0026-0495(13)00396-X
Knihovny.cz E-zdroje
- Klíčová slova
- Beige adipocyte, Brite adipocyte, Diabetes, Obesity,
- MeSH
- bílá tuková tkáň metabolismus MeSH
- dospělí MeSH
- feochromocytom genetika metabolismus MeSH
- fibroblastové růstové faktory genetika metabolismus MeSH
- hnědá tuková tkáň metabolismus MeSH
- hnědé tukové buňky metabolismus MeSH
- iontové kanály genetika metabolismus MeSH
- lidé středního věku MeSH
- lidé MeSH
- ligand 4-1BB genetika metabolismus MeSH
- mitochondriální proteiny genetika metabolismus MeSH
- nádory nadledvin genetika metabolismus MeSH
- novorozenec MeSH
- senioři MeSH
- transkripční faktory genetika metabolismus MeSH
- transkriptom genetika MeSH
- uncoupling protein 1 MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fibroblast growth factor 21 MeSH Prohlížeč
- fibroblastové růstové faktory MeSH
- iontové kanály MeSH
- ligand 4-1BB MeSH
- mitochondriální proteiny MeSH
- TNFSF9 protein, human MeSH Prohlížeč
- transkripční faktory MeSH
- UCP1 protein, human MeSH Prohlížeč
- uncoupling protein 1 MeSH
- ZIC1 protein, human MeSH Prohlížeč
OBJECTIVE: In rodents, brown (BAT) and white (WAT) adipose tissues are targets and expression sites for fibroblast growth factor-21 (FGF21). In contrast, human WAT expresses negligible levels of FGF21. We examined FGF21 expression in human BAT samples, including the induced BAT found in adult patients with pheochromocytoma, and interscapular and visceral BAT from newborns. METHODS: The expression of FGF21 and uncoupling protein-1 (UCP1, a brown adipocyte marker), was determined by quantitative real-time-PCR and immunoblotting. The transcript levels of marker genes for developmentally-programmed BAT (zinc-finger-protein of the cerebellum-1, ZIC1) and inducible-BAT (cluster of differentiation-137, CD137) were also determined. RESULTS: FGF21 and UCP1 are significantly expressed in visceral adipose tissue from pheochromocytoma patients, but not in visceral fat from healthy individuals. In neonates, FGF21 and UCP1 are both expressed in visceral and interscapular fat, and their expression levels show a significant positive correlation. Marker gene expression profiles suggest that inducible BAT is present in visceral fat from pheochromocytoma patients and neonates, whereas developmentally-programmed BAT is present in neonatal interscapular fat. CONCLUSIONS: Human BAT, but not WAT, expresses FGF21. The expression of FGF21 is especially high in inducible, also called beige/brite, neonatal BAT, but it is also found in the interscapular, developmentally-programmed, BAT of neonates.
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