Alkaloids from Chlidanthus fragrans and their acetylcholinesterase, butyrylcholinesterase and prolyl oligopeptidase activities
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
24427936
Knihovny.cz E-resources
- MeSH
- Acetylcholinesterase metabolism MeSH
- Amaryllidaceae Alkaloids chemistry isolation & purification metabolism MeSH
- Butyrylcholinesterase metabolism MeSH
- Liliaceae chemistry enzymology MeSH
- Magnetic Resonance Spectroscopy MeSH
- Prolyl Oligopeptidases MeSH
- Serine Endopeptidases metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Acetylcholinesterase MeSH
- Amaryllidaceae Alkaloids MeSH
- Butyrylcholinesterase MeSH
- Prolyl Oligopeptidases MeSH
- Serine Endopeptidases MeSH
Eleven Amaryllidaceae alkaloids (1-11) were isolated from fresh bulbs of Chlidanthus fragrans Herb. The chemical structures were elucidated by MS, and 1D and 2D NMR spectroscopic experiments. Complete NMR assignments were achieved for deoxypretazzetine (1). All compounds were evaluated for their erythrocytic acetylcholinesterase and serum butyrylcholinesterase inhibition activity using Ellman's method. In the prolyl oligopeptidase assay, Z-Gly-Pro-p-nitroanilide was used as substrate. In biological assays, only the crinine type Amaryllidaceae alkaloid undulatine showed promising acetylcholinesterase and prolyl oligopeptidase inhibition activity with IC50 values of 23.0 +/- 1.0 microM and 1.96 +/- 0.12 mM, respectively. Other isolated compounds were considered inactive.
Interactions of Isoquinoline Alkaloids with Transition Metals Iron and Copper
Amaryllidaceae Alkaloids as Potential Glycogen Synthase Kinase-3β Inhibitors
