OBJECTIVE: To evaluate the inflammatory pattern in maternal circulation, amniotic cavity, cervix and vagina from women with preterm prelabor rupture of membranes (PPROM) considering the occurrence of microbial invasion of the amniotic cavity (MIAC). METHODOLOGY: A prospective study was performed in 58 women with PPROM before 34+0 weeks of gestational age. Twenty-six proteins were analyzed by a multiple immunoassay in samples of amniotic fluid, serum, cervix and vagina. Association of an inflammatory response in the invasive and non-invasive samples with MIAC was investigated. RESULTS: The rate of MIAC was 36.2% (21/58). Both amniotic fluid IL-6 and cervical C-reactive protein (CRP) showed to be independent predictors of MIAC. A cut-off level of cervical CRP≥1836 pg/mL showed a detection rate of 75%, false positive rate of 19% and positive and negative predictive values to predict MIAC of 67% and 87%, respectively. There were no independent biomarkers of MIAC either in the serum or vaginal compartment. CONCLUSION: A cervical inflammatory response mediated by CRP was observed in PPROM women with MIAC. Evaluation of serum or vaginal samples did not add valuable information regarding the outcome evaluated.

Department of Clinical Biochemistry and Immunology Statens Serum Institut Copenhagen Denmark

Department of Obstetrics and Gynecology Sahlgrenska University Hospital Gothenburg Sweden ; Institute of Public Health Oslo Norway

Department of Obstetrics and Gynecology University Hospital Hradec Kralove Czech Republic

Maternal Fetal Medicine Department ICGON Hospital Clınic Universitat de Barcelona Barcelona Spain; Fetal and Perinatal Medicine Research Group Institut d'Investigacions Biomediques August Pi i Sunyer Barcelona Spain

PLoS One. 2017 Jan 20;12(1):e0170862. doi: 10.1371/journal.pone.0170862. PubMed

PLoS One. 2017 Oct 3;12(10):e0095905. doi: 10.1371/journal.pone.0095905. PubMed

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Maternal serum C-reactive protein concentration and intra-amniotic inflammation in women with preterm prelabor rupture of membranes

Maternal Serum C-Reactive Protein in Women with Preterm Prelabor Rupture of Membranes