Isoflavonoids in the Amaryllidaceae family
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Amaryllidaceae, ELISA, HPLC-ESI-MS, immunoaffinity chromatography, isoflavonoid,
- MeSH
- Alkaloids chemistry isolation & purification pharmacology MeSH
- Electron Spin Resonance Spectroscopy MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Genistein isolation & purification MeSH
- Isoflavones chemistry isolation & purification pharmacology MeSH
- Liliaceae chemistry MeSH
- Chromatography, High Pressure Liquid MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Alkaloids MeSH
- biochanin A MeSH Browser
- daidzein MeSH Browser
- Genistein MeSH
- Isoflavones MeSH
Plants of the Amaryllidaceae family are known as producers of biologically active alkaloids. Besides these a variety of flavonoids, including flavones, chalcones and chromones, have been detected in the Amaryllidaceous plants. In this study, we have analysed 16 representatives of the family for the presence of isoflavonoids. The water/ethanolic extracts were analysed with HPLC-ESI-MS both without any pre-treatment and after immunoaffinity chromatography as a clean-up step. Four individual immunosorbents specific for biochanin A, daidzein and genistein were used. In addition, five enzyme-linked immunosorbent assays specific for the above-mentioned isoflavonoids and their derivatives have been used for the analysis of the extracts after fractionation by semi-preparative HPLC. Fifteen selected isoflavonoids were detected in the studied samples, and the amount of individual compounds ranged between ca. 0.8 and 400 ng/g of dry weight. This study extends the number of known isoflavonoid-producing families within the monocotyledonous plants.
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